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SouthBayBio
NEDD8-AMC (human) (rec.) (untagged)
360
CHF
CHF 360.00
In stock
SBB-PS0044-C05050 µgCHF 360.00
Figure 1: Robustness of NEDD8-AMC substrates in an HTS format: Fluorescent substrate NEDD8-AMC was incubated with and without 30pM NEDP1 in a 384 well plate (n = 16), and progress curves were normalized to the maximum fluorescence signal to produce “% reaction progress”. The Z’ value, a statistical parameter widely used in the evaluation of screening assays, was calculated at each timepoint.
Figure 2: Signal to Background: The signal to background ratio was determined by 100% hydrolysis of 50nM NEDD8-AMC to liberate the quenched conjugate. Assay Buffer: 50mM HEPES pH7.5, 1mM TCEP, 0.1mg/ml BSA.
| Product Details | |
|---|---|
| Synonyms | Neural Precursor Cell Expressed Developmentally Down-Regulated 8; Neddylin |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Sequence |
Full-length, mature NEDD8 polypeptide conjugated on its C-terminus to AMC. |
| Crossreactivity | Human |
| Application |
Once hydrolyzed the free AMC provides excellent utility for real time assessment of enzyme activity at excitation (345nm) and emission (445nm). Typical working range is 50-500nM. |
| Label/Conjugates | AMC |
| MW | ~8.7kDa |
| Purity | ≥97% (LCMS) |
| Concentration | Lot dependent. |
| Accession Number | Q15843 |
| Formulation | Liquid. In 50mM MES pH 6.0, 150mM sodium chloride. |
| Other Product Data |
Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer |
| Declaration | Manufactured by South Bay Bio. |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -80°C |
| Long Term Storage | -80°C |
| Handling Advice | Aliquot to avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -80°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8) is a ubiquitin like protein that plays an important role in regulating development and the cell cycle. NEDD8 is conjugated to a target protein via a signaling cascade similar to ubiquitin: a NEDD8 specific E1 activating enzyme (APPBP1/UBA3) adenylates the c-terimnus of NEDD8 which is than subsequently passed to an E2 conjugating enzyme (UBE2M or UBE2F) that facilitates covalent attachment of NEDD8 to a cullin subunit of an SCF E3 ubiquitin ligase. NEDD8-Rh 110 can be used as a substrate for enzymes exhibiting deNEDDylating activity, e.g UCH-L3, UCH-L1, COP9 Signalosome and NEDP1. This product consists of a full-length, mature NEDD8 polypeptide (amino acids 1-76) recombinantly expressed in E.coli, conjugated on its C-terminus to a quenched AMC110 dye.
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