SouthBayBio

HA-Ubiquitin-vinyl methyl ester (human) (rec.) (HA)

CHF 366.00
In stock
SBB-PS0123-C05050 µgCHF 366.00
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Product Details
Synonyms HA-Ub-VME; HA-Ubiquitin-VME; HA-Ubiquitin-Vinyl methyl ester
Product Type Protein
Properties
Source/Host E. coli
Sequence

Human ubiquitin (aa1-76) (Accession Nr. P0CG47) with a C-terminal vinyl methyl ester group and a N-terminal HA-Tag.

Crossreactivity Human
Application

Typical working concentration range is 1-5 μM. Reaction conditions will need to be optimized for each specific application.

MW ~10kDa
Purity ≥97% (LCMS)
Concentration Lot dependent.
Accession Number P0CG47
Formulation Liquid. In 50mM MES pH 6.0, 100mM sodium chloride.
Other Product Data

Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer
Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by South Bay Bio.
Shipping and Handling
Shipping DRY ICE
Short Term Storage -20°C
Long Term Storage -80°C
Handling Advice Aliquot to avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -80°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF
Description

Ubiquitin is a small (8.5kDa) regulatory protein that has been found in almost all tissues of eukaryotic organisms. The addition of ubiquitin to a substrate protein is called ubiquitination or ubiquitylation. Ubiquitination can affect proteins in many ways: it can signal for their degradation via the proteasome, alter their cellular location, affect their activity and promote or prevent protein interactions. Removal of ubiquitin from a substrate protein occurs via deconjugating enzymes, of which there are nearly 100 known enzymes with various linkage specificities. This product consists of a full-length human, mature ubiquitin polypeptide (amino acids 1-76), expressed in E.coli with a C-terminal warhead (Vinyl Methyl Ester) and an N-terminal HA-Tag. HA-Ubiquitin vinyl methyl ester is a potent, irreversible and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic vinyl methyl ester group. It targets four of the five major DUB families: UCH (Ubiquitin C-terminal hydrolases), USP (Ubiquitin specific proteases), OTU (Ovarian tumor proteases) and MJD (Machado-Josephin domain proteases) while JAMM metalloproteases are not inhibited. HA-Ubiquitin vinyl methyl ester can be used for activity profiling experiments and using the HA-Tag conveniently for Immunoprecipitations in search for new or novel enzymes reacting with the c-terminal warhead.

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