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SouthBayBio
DDB1 (human) (rec.) (His) (Avi) (Biotin)
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Figure 1: His-Avi-DDB1 SDS-PAGE. From left to right, increasing amounts of protein loaded onto a 10-20% SDS-PAGE gel, stained with Coomassie brilliant blue. Purity is >97%.
| Product Details | |
|---|---|
| Synonyms | DNA Damage-binding Protein 1; DDB p127 Subunit; DNA Damage-binding Protein a (DDBa); HBV X-associated Protein 1 (XAP-1); UV-damaged DNA-binding Factor |
| Product Type | Protein |
| Properties | |
| Source/Host | Sf21 cells |
| Sequence |
Recombinant human DDB1 (aa 1-1140) with a N-terminal 8x-His Tag followed by an Avi-Tag that was endogenous biotinylated. |
| Crossreactivity | Human |
| MW | ~130kDa |
| Purity | ≥98% (SDS-PAGE) |
| Purity Detail | Biotinylation: >99% by Streptavidin-Bead pull-down. |
| Concentration | Lot dependent. |
| Accession Number | Q16531 |
| Formulation | Liquid. In 25mM HEPES pH 7.5, 150mM NaCl, 0.1mM TCEP (no detergents or glycerol). |
| Other Product Data |
Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer Our product description may differ slightly from the original manufacturer's product datasheet. |
| Declaration | Manufactured by South Bay Bio. |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -80°C |
| Long Term Storage | -80°C |
| Handling Advice | Aliquot to avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -80°C. |
| Documents | |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
DNA damage-binding protein 1, which is involved in both DNA repair and protein ubiquitination, is part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively. Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruits proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. The protein has an N-terminal 8x-His Tag followed by an Avi-Tag that was endogenously biotinylated during expression in Sf21 Cells.
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