Antibody Oligonucleotide All-in-One Conjugation Kit

Oligonucleotide-Antibody Labeling

Affinity Magnetic Beads, S-HyNic Crosslinker (DMF Soluble), Modification Buffer (10X), Anhydrous DMF, Red Cap Spin Column, Yellow Cap Spin Column, Brown Cap Spin Column, Blue Cap Spin Column, Oligo Resuspension Solution, Bead Wash Buffer, Bead Elution Buffer, S-4FB Crosslinker (DMF Soluble), 2- Hydrazinopyridine dihydrochloride, 2 ml Collection tubes.

Click here for Original Manufacturer Product Datasheet and Complete Product Information

The Antibody-Oligonucleotide All-in-One Conjugation kit uses Solulink’s patented conjugation chemistry to link an antibody to an oligonucleotide. The first stage of the process begins with the labeling of a 3’ or 5’- amino-modified oligonucleotide with S-4FB. This amine-reactive NHS ester incorporates an aromatic aldehyde functional group, formylbenzamide (4FB), at the desired terminus of the oligonucleotide. In stage two of the process, a polyclonal or monoclonal antibody (100 µg) is modified using the complementary linker S-HyNic. This NHS-ester reacts with lysine residues, incorporating HyNic functional groups (hydrazinonicotinamide) onto the antibody. In the third and final stage, the two modified biomolecules are mixed together in the presence of a reaction catalyst (aniline) to form the conjugate, after which purification is carried out using a magnetic affinity solid phase.