ChromaLink Digoxigenin One-Shot Antibody Labeling Kit

Modification Buffer (10X), Anhydrous DMF, 1X PBS, Zeba Desalting Colums, Sulfo-ChromaLink Digoxigenin, 1.5 ml Collection Tubes, DIG-Labeled Bovine IgG Control, 1M Tris HCl.

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The ChromaLink Digoxigenin One-Shot kit is a convenient, cost-effective way of incorporating digoxigenin into a defined quantity of antibody. After incorporation, digoxigenin is rapidly quantified using a non-destructive UV-scan (220-400 nm). The kit features high antibody recoveries (50-80%) with consistent digoxigenin incorporation. The degree of incorporation or molar substitution ratio (MSR) ranges from 2-8 digoxigenin molecules per antibody when used as directed. The One-Shot kit can be used to label a diverse group of antibodies including all mammalian IgG, IgE, and avian IgY molecules. The One-Shot kit offers a robust method of both labeling and quantifying incorporated digoxigenin with a simple protocol. This “double-feature” provides researchers with greater confidence, consistency and reproducibility in all downstream applications. 

Applications: Antibody Labeling, Antisense/RNAi, Aptamers, Immunotherapeutics, In Situ Proximity Ligation, In vitro Diagnostic (IVD), Laboratory Developed Tests (LDT), Analyte Specific Reagents (ASR)