ChromaLink One-Shot Antibody Biotinylation Kit

Collection Tubes, Modification Buffer (10X), Anhydrous DMF, ChromaLink^® Biotin (DMF Soluble), 1X PBS, Biotinylated Bovine IgG Control, Zeba Desalting Colums, 1.5 ml storage tubes, 2 ml Collection tubes, 1M Tris HCl, 10X PBS, Bovine IgG, Biotinylated Bovine IgG.

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The ChromaLink Biotin One-Shot Kit is a simple, cost-effective way of incorporating a readily verifiable amount of biotin into a defined quantity of antibody. The kit conveniently features a pre-weighed amount of amine-reactive ChromaLink™ Biotin reagent that is readily resuspended directly into the antibody solution. It can be used to label a variety of different antibodies, including mammalian IgG (monoclonal or polyclonal) and/or avian IgYs. Biotin incorporation is rapidly determined by means of a simple, non-destructive UV-scan (220–400 nm) of the sample after removal of excess labeling reagent. The kit features high antibody recovery (60–90 µg) and a consistent level of biotin incorporation (3–8 biotin molecules per antibody molecule) when used as directed.

Applications: Antibody Labeling, Antisense/RNAi, Aptamers, Immunotherapeutics, In Situ Proximity Ligation, In vitro Diagnostic (IVD), Laboratory Developed Tests (LDT), Analyte Specific Reagents (ASR)