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Vector
ChromaLink Biotin Protein Labeling Kit
Product Details | |
---|---|
Product Type | Kit |
Properties | |
Application Set | Labeling |
Kit Contains |
ChromaLink Biotin (5 x 0.5 mg), 10X Modification Buffer (2 x 1.5 mL), 10X PBS (2 x 1.5 mL), 0.5 mL Zeba™ Column (12), 2 mL Collection Tube (2 x 12), 1.5 mL Storage Tube (12), Bovine IgG Control (0.5 mg), Biotinylated Bovine IgG (0.5 mg), Anhydrous DMF (1.5 mL) |
Other Product Data |
Click here for Original Manufacturer Product Datasheet and Complete Product Information ChromaLink Biotin is the most advanced protein biotinylation reagent available. It features a succinimidyl ester functional group, which modifies protein lysine residues in an aqueous buffer. The linker is UV-traceable, and absorbs at 354 nm. This UV-signature permits rapid quantification of incorporated biotin, by means of nondestructive measurement (A280/A354 nm). The linker possesses an extended PEG3 spacer, which helps preserve and maintain streptavidin/biotin affinity. This kit is used to biotinylate antibodies, proteins and peptides greater than 7kDa for the use in Western Blot, ELISA, immobilization and immunohistochemistry applications. May also be used in any assay as an alternative to a standard NHS-Biotin linker. Applications: Antibody Labeling, Antisense/RNAi, Aptamers, Immunotherapeutics, In Situ Proximity Ligation, In vitro Diagnostic (IVD), Laboratory Developed Tests (LDT), Analyte Specific Reagents (ASR) |
Declaration | Manufactured by Vector Laboratories |
Shipping and Handling | |
Shipping | AMBIENT |
Short Term Storage | +20°C |
Long Term Storage | +4°C |
Handling Advice | Do not freeze. |
Documents | |
Manual | Download PDF |
MSDS | Download PDF |
Product Specification Sheet | |
Datasheet | Download PDF |
The ChromaLINK Biotin Protein Labeling Kit includes everything you need to biotinylate your protein: buffers, reagents, spin columns, and an easy-to-follow protocol and online calculator. With the ChromaLINK Biotin Protein Labeling Kit you can biotinylate and purify up to 5 antibodies or other proteins ranging in molecular weight from 20–200 kDa and from as little as 25 µg to as much as 1 mg.