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AdipoGen Life Sciences

anti-NLRP3/NALP3, mAb (Cryo-2) (Biotin)

CHF 280.00
In stock
AG-20B-0014B-C05050 µgCHF 280.00
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Figure 1: Mouse NLRP3 is detected in mouse macrophages using the non-biotinylated monoclonal antibody to NLRP3 (Cryo-2) (Prod. No. AG-20B-0014). Method: Cell extracts from mouse macrophages (BMDMs) WT +/+ (lane 1), NLRP3+/- (lane 2) or NLRP3 -/- (lane 3), with or without treatment with LPS (50ng/ml) for 3h, were separated by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the mAb to NLRP3 (Cryo-2) (1µg/ml). Proteins are visualized by a chemiluminescence detection system.
Picture courtesy of Dr. Olaf Gross, Munich University, Germany.
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Product Details
Synonyms NACHT-, LRR- and PYD-containing Protein 3; NLRP3; Cryopyrin; Cold Autoinflammatory Syndrome 1 Protein
Product Type Monoclonal Antibody
Properties
Clone Cryo-2
Isotype Mouse IgG2b
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Recombinant mouse NLRP3/NALP3 (pyrin domain/aa 1-93).
Label/Conjugates Biotin
Application

Western Blot (see online protocol): (1μg/ml)
Immunocytochemistry: (see J. Virol. 85, 13019 (2011) and Nat. Immunol. 13, 255 (2012))
Immunoprecipitation: (in RIPA buffer, see Mol. Cell 49, 331 (2013))
Immunohistochemistry: (1:200, for a protocol see Nature 493, 67 (2012))
ChIP assay: 2µg/DNA of 10E6 cells (see Nat. Immunol. 16, 859 (2015))
Crossreactivity Human
Mouse
Specificity

Recognizes mouse and human NLRP3/NALP3.
Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS containing 0.02% Proclin300.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
Protocols Download PDF
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

The inflammasome is a multiprotein complex that mediates the activation of caspase-1, which promotes, amongst others, the secretion of the proinflammatory cytokines interleukin (IL)-1β and IL-18. Members of the Nod-like receptor family, including NLRP3/NALP3, are critical components of the inflammasome that link danger-signals to caspase-1 activation. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1), Muckle-Wells syndrome (MWS) and of chronic infantile neurologic cutaneous and articular syndrome (CINCA).

Product References
  1. Thioredoxin-interacting protein links oxidative stress to inflammasome activation: R. Zhou, et al.; Nature Immunology 11, 136 (2010)
  2. The NLRP3 inflammasome promotes renal inflammation and contributes to CKD: A. Vilaysane, et al.; J. Am. Soc. Nephrol. 21, 1732 (2010)
  3. A role for mitochondria in NLRP3 inflammasome activation: R. Zhou, et al.; Nature 469, 221 (2011)
  4. Differential expression of NLRP3 among hematopoietic cells: G. Guarda, et al.; J. Immunol. 186, 2529 (2011)
  5. Mechanism of Impaired NLRP3 Inflammasome Priming by Monophosphoryl Lipid A: C.A. Embry, et al.; Sci. Signal. 4, ra28 (2011)
  6. Measles Virus V Protein Inhibits NLRP3 Inflammasome-Mediated Interleukin-1 Secretion: N. Komune, et al.; J. Virol. 85, 13019 (2011)
  7. Novel role of PKR in inflammasome activation and HMGB1 release: B. Lu, et al.; Nature 488, 670 (2012)
  8. Deubiquitination of NLRP3 by BRCC3 Critically Regulates Inflammasome Activity: B.F. Py, et al.; Mol. Cell 49, 331 (2013)
  9. NLRP3 is activated in Alzheimer's disease and contributes to pathology in APP/PS1 mice: M.T. Heneka, et al.; Nature 493, 67 (2012)
  10. Activation of autophagy by inflammatory signals limits IL-1b production by targeting ubiquitinated inflammasomes for destruction: C.-S. Shi, et al.; Nat. Immunol. 13, 255 (2012)
  11. Inflammasome-independent NLRP3 augments TGF-β signaling in kidney epithelium: W. Wang, et al.; J. Immunol. 190, 1239 (2013)
  12. Omega-3 Fatty Acids Prevent Inflammation and Metabolic Disorder through Inhibition of NLRP3 Inflammasome Activation: Y. Yan, et al.; Immunity 38, 1154 (2013)
  13. LRRFIP2 negatively regulates NLRP3 inflammasome activation in macrophages by promoting Flightless-I-mediated caspase-1 inhibition: J. Jin, et al.; Nat. Commun. 4, 2075 (2013)
  14. Proapoptotic Chemotherapeutic Drugs Induce Noncanonical Processing and Release of IL-1β via Caspase-8 in Dendritic Cells: C. Antonopoulus, et al. J. Immunol. 191, 4789 (2013)
  15. Constitutively activated NLRP3 inflammasome causes inflammation and abnormal skeletal development in mice: S.L. Bonar, et al.; PLoS One 7, e35979 (2012)
  16. FADD and caspase-8 mediate priming and activation of the canonical and noncanonical Nlrp3 inflammasomes: P. Gurung, et al.; J. Immunol. 192, 1835 (2014)
  17. XIAP Restricts TNF- and RIP3-Dependent Cell Death and Inflammasome Activation: M. Yabal, et al.; Cell Rep. 7, 1796 (2014)
  18. The adaptor ASC has extracellular and 'prionoid' activities that propagate inflammation: B.S. Franklin, et al.; Nat. Immunol. 15, 727 (2014)
  19. The NLRP3 inflammasome is released as a particulate danger signal that amplifies the inflammatory response: A. Baroja-Mazo, et al.; Nat. Immunol. 15, 738 (2014)
  20. Toward a chemical marker for inflammatory disease: a fluorescent probe for membrane-localized thioredoxin: M.H. Lee, et al.; JACS 136, 8430 (2014)
  21. Prostaglandin E2 inhibits NLRP3 inflammasome activation through EP4 receptor and intracellular cyclic AMP in human macrophages: M. Sokolowska, et al.; J. Immunol. 194, 5472 (2015)
  22. The receptor NLRP3 is a transcriptional regulator of TH2 differentiation: M. Bruchard, et al.; Nat. Immunol. 16, 859 (2015)
  23. NLRP3 Inflammasome Is Expressed and Functional in Mouse Brain Microglia but Not in Astrocytes: A. Gustin, et al.; PLos One 10, e0130624 (2015)
  24. Nlrp6 promotes recovery after peripheral nerve injury independently of inflammasomes: E. Ydens, et al.; J. Neuroinfl. 12, 143 (2015)
  25. Human monocytes engage an alternative inflammasome pathway: M.M. Gaidt, et al.; Immunity 44, 833 (2016)
  26. Measuring NLR Oligomerization I: Size Exclusion Chromatography, Co-immunoprecipitation, and Cross-Linking: S. Khare, et al.; Methods Mol. Biol. 1417, 131 (2016)
  27. Functional Reconstruction of NLRs in HEK293 Cells: V. Compan & G. Lopez-Castejon; Methods Mol. Biol. 1417, 217 (2016)
  28. The NLRP3 inflammasome and bruton's tyrosine kinase in platelets co-regulate platelet activation, aggregation, and in vitro thrombus formation: P. Murthy, et al.; BBRC 483, 230 (2017)
  29. Pioglitazone Confers Neuroprotection Against Ischemia-Induced Pyroptosis due to its Inhibitory Effects on HMGB-1/RAGE and Rac1/ROS Pathway by Activating PPAR-γ: P. Xia, et al.; Cell Physiol. Biochem. 45, 2351 (2018)
  30. The Crohn’s disease risk factor IRGM limits NLRP3 inflammasome activation by impeding its assembly and by mediating its selective autophagy: S. Mehto, et al.; Mol. Cell 73, 429 (2019)
  31. HSP70 is a negative regulator of NLRP3 inflammasome activation: P. Martine, et al.; Cell Death Diff. 10, 256 (2019)
  32. Lack of Protein Prenylation Promotes Nlrp3 Inflammasome Assembly in Human Monocytes: O.P. Skinner, et al.; J. Allergy Clin. Immunol. 143, 2315(2019) [KO validation]
  33. Cathepsin B Is Required for NLRP3 Inflammasome Activation in Macrophages, Through NLRP3 Interaction: A. Chevriaux, et al.; Front. Cell. Dev. Biol. 8, 167 (2020)
  34. Caspase-6 Is a Key Regulator of Innate Immunity, Inflammasome Activation, and Host Defense: M. Zheng, et al.; Cell 181, 674 (2020)
  35. Oxidized DNA fragments exit mitochondria via mPTP- and VDAC-dependent channels to activate NLRP3 inflammasome and interferon signaling: H. Xian, et al.; Immunity 55, 1370 (2022)
  36. HSP90beta controls NLRP3 autoactivation: L. Spel, et al.; Sci. Adv. 10, eadj6289 (2024)
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