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AdipoGen Life Sciences
anti-Caspase-1 (p20) (human), mAb (Bally-1)
480
CHF
CHF 480.00
In stock
AG-20B-0048-C100100 µgCHF 480.00
Figure 1: Human Caspase-1 (p20) is detected by immunoblotting using anti-Caspase-1 (p20) (human), mAb (Bally-1) (Prod. No AG-20B-0048).
Method: Caspase-1 was analyzed by Western blot in supernatants of THP1 cells differentiated for 3h with 0.5 µM PMA (Prod. No. AG-CN2-0010) and activated (lane 2) or not (lane 1) by 5 µM Nigericin for 1h (Prod. No. AG-CN2-0020). Supernatants (30µl) were separated by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with anti-Caspase-1 (p20) (human), mAb (Bally-1) (1µg/ml). Proteins were visualized by a chemiluminescence detection system.
Method: Caspase-1 was analyzed by Western blot in supernatants of THP1 cells differentiated for 3h with 0.5 µM PMA (Prod. No. AG-CN2-0010) and activated (lane 2) or not (lane 1) by 5 µM Nigericin for 1h (Prod. No. AG-CN2-0020). Supernatants (30µl) were separated by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with anti-Caspase-1 (p20) (human), mAb (Bally-1) (1µg/ml). Proteins were visualized by a chemiluminescence detection system.
| Product Details | |
|---|---|
| Synonyms | Interleukin-1 β Convertase; IL-1BC; Interleukin-1 β-converting Enzyme; ICE |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | Bally-1 |
| Isotype | Mouse IgG1 |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Recombinant human caspase-1. |
| Application |
Western Blot (see online protocol): (1μg/ml) Note: Depending on the used cell line we recommend to optimize the dilution. |
| Crossreactivity | Human |
| Specificity |
Recognizes endogenous full-length and activated (p20 fragment) human caspase-1. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| Protocols |
 Download PDF |
| MSDS |
Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1β (IL-1β) and IL-18 and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called Inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3 responds to a variety of agonists including crystals.
Product References
- Measuring the inflammasome: O. Gross; Methods Mol. Biol. 844, 199 (2012)
- Liver X receptor β activation induces pyroptosis of human and murine colon cancer cells: V. Derangere, et al.; Cell Death Differ. 21, 1914 (2014)
- Caspase-11 cleaves gasdermin D for non-canonical inflammasome signaling: N. Kayagaki, et al.; Nature 526, 666 (2015)
- Human monocytes engage an alternative inflammasome pathway: M.M. Gaidt, et al.; Immunity 44, 833 (2016)
- Cell-Free Assay for Inflammasome Activation: Y. Jamilloux & F. Martinon; Methods Mol. Biol. 1417, 207 (2016)
- The TAT-RasGAP317-326 anti-cancer peptide can kill in a caspase-, apoptosis-, and necroptosis-independent manner: M. Heulot, et al.; Oncotarget 7, 6342 (2016)
- Impact of human monocyte and macrophage polarization on NLR expression and NLRP3 inflammasome activation: F. Awad, et al.; PLoS ONE 12, e0175336 (2017)
- The DNA inflammasome in human myeloid cells is initiated by a STING-cell death program upstream of NLRP3: M.M. Gaidt, et al.; Cell 171, 1110 (2017)
- Calcium phosphate particles stimulate interleukin-1β release from human vascular smooth muscle cells: A role for spleen tyrosine kinase and exosome release: Y. Dautova, et al.; J. Mol. Cell Cardiol. 115, 82 (2017)
- POH1 deubiquitinates pro-interleukin-1β and restricts inflammasome activity: L. Zhang, et al.; Nat. Commun. 9, 4225 (2018)
- SUMO-mediated regulation of NLRP3 modulates inflammasome activity: R. Barry, et al.; Nat. Commun. 9, 3001 (2018)
- The Crohn’s disease risk factor IRGM limits NLRP3 inflammasome activation by impeding its assembly and by mediating its selective autophagy: S. Mehto, et al.; Mol. Cell 73, 429 (2019)
- Human GBP1 is a microbe-specific gatekeeper of macrophage apoptosis and pyroptosis: D. Fisch, et al.; EMBO J. 38, e100926 (2019)
- HSP70 is a negative regulator of NLRP3 inflammasome activation: P. Martine, et al.; Cell Death Diff. 10, 256 (2019)
- Inflammasome Activation Triggers Blood Clotting and Host Death through Pyroptosis: C. Wu, et al.; Immunity 50, 1401 (2019)
- SERPINB1-mediated checkpoint of inflammatory caspase activation: Y.J. Choi, et al.; Nat. Immunol. 20, 276 (2019) [Knock Down Validation]
- NLRP3 inflammasome activation drives tau pathology: C. Ising, et al.; Nature 575, 669 (2019)
- Human GBP1 Differentially Targets Salmonella and Toxoplasma to License Recognition of Microbial Ligands and Caspase-Mediated Death: D. Fisch, et al.; Cell Rep. 32, 108008 (2020)
- Molecularly Distinct NLRP3 Inducers Mediate Diverse Ratios of Interleukin-1β and Interleukin-18 from Human Monocytes: K. Midtbo, et al.; Med. Inflamm. ID4651090 (2020)
- Cross-regulation between LUBAC and caspase-1 modulates cell death and inflammation: T. Douglas & M. Saleh; J. Biol. Chem. 295, 5216 (2020)
- AIM2 Inhibits BRAF-Mutant Colorectal Cancer Growth in a Caspase-1-Dependent Manner: S. Shah, et al.; Front. Cell Dev. Biol. 9, 588278 (2021)
- Human NLRP1 is a sensor for double-stranded RNA: S. Bauernfried, et al.; Science 371, 482 (2021)
- Post-injury immunosuppression and secondary infections are caused by an AIM2 inflammasome-driven signaling cascade: S. Roth, et al.; Immunity 54, 648 (2021)
- HSP90beta controls NLRP3 autoactivation: L. Spel, et al.; Sci. Adv. 10, eadj6289 (2024)
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