anti-Caspase-1 (p20) (human), mAb (Bally-1)
Method: Caspase-1 was analyzed by Western blot in supernatants of THP1 cells differentiated for 3h with 0.5 µM PMA (Prod. No. AG-CN2-0010) and activated (lane 2) or not (lane 1) by 5 µM Nigericin for 1h (Prod. No. AG-CN2-0020). Supernatants (30µl) were separated by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with anti-Caspase-1 (p20) (human), mAb (Bally-1) (1µg/ml). Proteins were visualized by a chemiluminescence detection system.
|Synonyms||Interleukin-1 β Convertase; IL-1BC; Interleukin-1 β-converting Enzyme; ICE|
|Product Type||Monoclonal Antibody|
|Source/Host||Purified from concentrated hybridoma tissue culture supernatant.|
|Immunogen/Antigen||Recombinant human caspase-1.|
Western Blot (see online protocol): (1μg/ml)
Note: Depending on the used cell line we recommend to optimize the dilution.
Recognizes endogenous full-length and activated (p20 fragment) human caspase-1.
|Purity Detail||Protein G-affinity purified.|
|Formulation||Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
|Use/Stability||Stable for at least 1 year after receipt when stored at -20°C.|
|Product Specification Sheet|
Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1β (IL-1β) and IL-18 and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called Inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3 responds to a variety of agonists including crystals.
- Measuring the inflammasome: O. Gross; Methods Mol. Biol. 844, 199 (2012)
- Liver X receptor β activation induces pyroptosis of human and murine colon cancer cells: V. Derangere, et al.; Cell Death Differ. 21, 1914 (2014)
- Caspase-11 cleaves gasdermin D for non-canonical inflammasome signaling: N. Kayagaki, et al.; Nature 526, 666 (2015)
- Human monocytes engage an alternative inflammasome pathway: M.M. Gaidt, et al.; Immunity 44, 833 (2016)
- Cell-Free Assay for Inflammasome Activation: Y. Jamilloux & F. Martinon; Methods Mol. Biol. 1417, 207 (2016)
- Impact of human monocyte and macrophage polarization on NLR expression and NLRP3 inflammasome activation: F. Awad, et al.; PLoS ONE 12, e0175336 (2017)
- The DNA inflammasome in human myeloid cells is initiated by a STING-cell death program upstream of NLRP3: M.M. Gaidt, et al.; Cell 171, 1110 (2017)
- Calcium phosphate particles stimulate interleukin-1β release from human vascular smooth muscle cells: A role for spleen tyrosine kinase and exosome release: Y. Dautova, et al.; J. Mol. Cell Cardiol. 115, 82 (2017)
- The Crohn’s disease risk factor IRGM limits NLRP3 inflammasome activation by impeding its assembly and by mediating its selective autophagy: S. Mehto, et al.; Mol. Cell 73, 429 (2019)
- SERPINB1-mediated checkpoint of inflammatory caspase activation: Y.J. Choi, et al.; Nat. Immunol. 20, 276 (2019) [Knock Down Validation]