AdipoGen Life Sciences

anti-Caspase-1 (p20) (human), mAb (Bally-1)

CHF 480.00
In stock
AG-20B-0048-C100100 µgCHF 480.00
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Product Details
Synonyms Interleukin-1 β Convertase; IL-1BC; Interleukin-1 β-converting Enzyme; ICE
Product Type Monoclonal Antibody
Clone Bally-1
Isotype Mouse IgG1
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Recombinant human caspase-1.

Western Blot (see online protocol): (1μg/ml)

Note: Depending on the used cell line we recommend to optimize the dilution.

Crossreactivity Human

Recognizes endogenous full-length and activated (p20 fragment) human caspase-1.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Isotype Negative Control

Mouse IgG1 Isotype Control

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Protocols Download PDF
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1β (IL-1β) and IL-18 and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called Inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3 responds to a variety of agonists including crystals.

Product References
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  2. Liver X receptor β activation induces pyroptosis of human and murine colon cancer cells: V. Derangere, et al.; Cell Death Differ. 21, 1914 (2014)
  3. Caspase-11 cleaves gasdermin D for non-canonical inflammasome signaling: N. Kayagaki, et al.; Nature 526, 666 (2015)
  4. Human monocytes engage an alternative inflammasome pathway: M.M. Gaidt, et al.; Immunity 44, 833 (2016)
  5. Cell-Free Assay for Inflammasome Activation: Y. Jamilloux & F. Martinon; Methods Mol. Biol. 1417, 207 (2016)
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  9. Calcium phosphate particles stimulate interleukin-1β release from human vascular smooth muscle cells: A role for spleen tyrosine kinase and exosome release: Y. Dautova, et al.; J. Mol. Cell Cardiol. 115, 82 (2017)
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  12. The Crohn’s disease risk factor IRGM limits NLRP3 inflammasome activation by impeding its assembly and by mediating its selective autophagy: S. Mehto, et al.; Mol. Cell 73, 429 (2019)
  13. Human GBP1 is a microbe-specific gatekeeper of macrophage apoptosis and pyroptosis: D. Fisch, et al.; EMBO J. 38, e100926 (2019)
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  18. Human GBP1 Differentially Targets Salmonella and Toxoplasma to License Recognition of Microbial Ligands and Caspase-Mediated Death: D. Fisch, et al.; Cell Rep. 32, 108008 (2020)
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  20. Cross-regulation between LUBAC and caspase-1 modulates cell death and inflammation: T. Douglas & M. Saleh; J. Biol. Chem. 295, 5216 (2020)
  21. AIM2 Inhibits BRAF-Mutant Colorectal Cancer Growth in a Caspase-1-Dependent Manner: S. Shah, et al.; Front. Cell Dev. Biol. 9, 588278 (2021)
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