anti-Fas (human), mAb (APO-1-1) (preservative free)

CHF 240.00
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AG-20B-0079PF-C100100 µgCHF 240.00
AG-20B-0079PF-C500500 µgCHF 750.00
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Product Details
Synonyms CD95; APO-1; TNFRSF6; Tumor Necrosis Factor Receptor Superfamily Member 6; Apoptosis-mediating Surface Antigen FAS
Product Type Monoclonal Antibody
Properties
Clone APO-1-1
Isotype Mouse IgG1
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Recombinant human Fas.
Application

Flow Cytometry 1-2µg/ml
Functional Application: Induces apoptosis upon cross-linking.
Immunohistochemistry: Frozen sections; not suitable for stainings on paraffin sections.

Note: APO-1-1 (IgG1) is an isotype-switch variant of the original clone APO-1-3 (IgG3) (Prod. No. AG-20B-0062PF). Since undesired side reactions in immunohistochemistry are minimal for IgG1 antibodies, APO-1-1 is especially useful for immunohistology on cryostat sections and on cytospin preparations. For Western Blot use anti-Fas (human), mAb (APO-1-3) (Prod. No. AG-20B-0062PF).

Crossreactivity Human
Specificity

Recognizes human Fas.

Purity ≥95% (SDS-PAGE)
Concentration 1 mg/ml.
Formulation Liquid. In PBS.
Other Product Data

Uniprot: Fas (human)

Accession Number P25445
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

Fas (CD95) is a member of the death receptor (DR) family, a subfamily of the tumor necrosis factor receptor superfamily. The formation of the Fas death-inducing signaling complex (DISC) is the initial step of Fas signaling. Activation of procaspase-8 at the DISC leads to the induction of DR-mediated apoptosis. Stimulation of Fas has also been reported to trigger non-apoptotic pathways. It has been shown that membrane-bound FasL is essential for the cytotoxic activity, whereas soluble FasL appears to promote autoimmunity and tumorigenesis via induction of non-apoptotic pathways, in particular NF-κB.

Product References
  1. Monoclonal antibody-mediated tumor regression by induction of apoptosis: B.C. Trauth, et al.; Science 245, 301 (1989)
  2. Induction of apoptosis by monoclonal antibody anti-APO-1 class switch variants is dependent on cross-linking of APO-1 cell surface antigens: J. Dhein, et al.; J. Immunol. 149, 3166 (1992)
  3. Constitutive and induced expression of APO-1, a new member of the nerve growth factor/tumor necrosis factor receptor superfamily, in normal and neoplastic cells: F. Leithauser, et al.; Lab. Invest. 69, 415 (1993) (IHC Application)
  4. Ligand-independent redistribution of Fas (CD95) into lipid rafts mediates clonotypic T cell death: J.R. Muppidi & R.M. Siegel; Nat. Immunol. 5, 182 (2004)
  5. Role of CD95-mediated adipocyte loss in autoimmune lipodystrophy: P. Fischer-Posovszky, et al.; J. Clin. Endocrinol. Metab. 91, 1129 (2006)
  6. Cutting edge: Rac GTPases sensitize activated T cells to die via Fas: M. Ramaswamy, et al.; J. Immunol. 179, 6384 (2007)
  7. Low FAS/CD95 Expression by CTCL Correlates withReduced Sensitivity to Apoptosis that Can BeRestored by FAS Upregulation: J. Wu, et al.; J. Invest. Dermatol. 129, 1165 (2009) (IHC Application)
  8. Natural killer cells induce distinct modes of cancer cell death: Discrimination, quantification and modulation of apoptosis, necrosis and mixed forms: C.S. Backes, et al.; J. Biol. Chem. 293, 16348 (2018)
  9. Epigenetically Enhanced PDT Induces Significantly Higher Levels of Multiple Extrinsic Pathway Apoptotic Factors than Standard PDT, Resulting in Greater Extrinsic and Overall Apoptosis of Cutaneous T-cell Lymphoma: K.A. Salva, et al.; Photochem. Photobiol. 94, 1058 (2018) (IHC Application)
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