anti-TNF-α (mouse), mAb (blocking) (V1q) (preservative free)

CHF 360.00
In stock
AG-20B-0081PF-C100100 µgCHF 360.00
AG-20B-0081PF-C500500 µgCHF 1'080.00
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Product Details
Synonyms Tumor Necrosis Factor Ligand Superfamily Member 2; TNFSF2; Cachectin
Product Type Monoclonal Antibody
Properties
Clone V1q
Isotype Rat IgG
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Cytotoxin purified from ConA induced T cell clone 29.
Label/Conjugates Preservative Free
Application

Functional Application: Inhibition of mouse TNF-α function in vivo (in mice). 20µg of antibody/mouse is sufficient to block the activity of endogenous mouse TNF-α.
Flow Cytometry: (1:200-1:1'000)

Crossreactivity Mouse
Specificity

Recognizes mouse TNF-α. Reacts with both natural and recombinant mouse TNF-α. It does not cross-react with human TNF-α and TNF-β (Lymphotoxin-α).

Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified.
Endotoxin Content <0.01EU/μg purified protein (LAL test).
Concentration 1mg/ml
Formulation Liquid. In PBS.
Isotype Negative Control

Rat IgG1 Isotype Control (preservative free)

Accession Number P06804
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Tumor necrosis factor (TNF, cachexin or cachectin and formerly known as tumor necrosis factor-α) is a cytokine involved in systemic inflammation and metabolism and is a member of a group of cytokines that stimulate the acute phase reaction. It causes tumor necrosis in vivo and shows also cytolytic activity against tumors. Signal transduction occurs via two types of TNF-receptors, the TNF-receptors 1 and 2. The receptor TNF-R1 is activated by both the membrane-bound and soluble trimeric forms of TNF-α, whereas the receptor TNF-R2 only responds to the membrane-bound form of TNF-α. The monoclonal antibody V1q recognizes endogenous mouse TNF-α.

Product References
  1. Requirement of endogenous tumor necrosis factor/cachectin for recovery from experimental peritonitis: B. Echtenacher, et al.; J. Immunol. 145, 3762 (1990)
  2. Pharmacologic Inhibitors of Tumor Necrosis Factor Production Exert Differential Effects in Lethal Endotoxemia and in Infection with Live Microorganisms in Mice: M.G. etea, et al.; J. Inf. Dis. 171, 393 (1995)
  3. A monoclonal antibody against tumour necrosis factor-alpha improves survival in experimental multiple organ dysfunction syndrome: M.J. Jansen, et al.; Cytokine 10, 904 (1998)
  4. Anti-inflammatory effects of systemic anti-tumour necrosis factor α treatment in human/murine SCID arthritis: H. Schadlich, et al.; Ann. Rheum. Dis. 58, 428 (1999)
  5. Therapeutic neutralization of CD95-ligand and TNF attenuates brain damage in stroke: A. Martin-Villablba, et al.; Cell Death Diff. 8, 679 (2001)
  6. Roles of tumor necrosis factor p55 and p75 receptors in TNF-a-induced vascular permeability: E. Ferrero, et al.; Am. J. Physiol. Cell Physiol. 281, C1173 (2001)
  7. Improving chemotherapeutic drug penetration in tumors by vascular targeting and barrier alteration: F. Curnis, et al.; J. Clin. Invest. 110, 475 (2002)
  8. An Anti-Tumor Necrosis Factor-α Antibody Inhibits the Development of Experimental Skin Tumors: K.A. Scott, et al.; Mol. Cancer Therap. 2, 445 (2003)
  9. Neutralization of CD95 ligand promotes regeneration and functional recovery after spinal cord injury: D. Demjen, et al.; Nat. Med. 10, 389 (2004)
  10. Blockade of TNF-α Decreases Both Inflammation and Efficacy of Intrapulmonary Ad.IFNβ Immunotherapy in an Orthotopic Model of Bronchogenic Lung Cancer: M.J. Wilderman, et al.; Mol. Ther. 13, 910 (2006)
  11. Severely impaired insulin signaling in chronic wounds of diabetic ob/ob mice: a potential role of tumor necrosis factor-alpha: I. Goren, et al.; Am. J. Pathol. 168, 765 (2006)
  12. Systemic Anti-TNFα Treatment Restores Diabetes-Impaired Skin Repair in ob/ob Mice by Inactivation of Macrophages: I. Goren, et al.; J. Clin. Invest. 127, 2259 (2007)
  13. Tumor Necrosis Factor a Sensitizes Primary Murine Hepatocytes to Fas/CD95-Induced Apoptosis in a Bim- and Bid-Dependent Manner: K. Schmich, et al.; Hepatol. 53, 282 (2011)
  14. TNF signaling drives myeloid-derived suppressor cell accumulation: X. Zhao, et al.; J. Clin. Invest. 122, 4094 (2012)
  15. Long-term amelioration of established collagen-induced arthritis achieved with short-term therapy combining anti-CD3 and anti-tumor necrosis factor treatments: F. Depis, et al.; Arthr. Rheumatol. 64, 3189 (2012)
  16. Targeting Endothelial CD146 Attenuates Colitis and Prevents Colitis-Associated Carcinogenesis: S. Xing, et al.; Am. J. Pathol. 184, 1604 (2014)
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