AdipoGen Life Sciences

anti-Caspase-11 (mouse), mAb (8A5)

CHF 430.00
In stock
AG-20T-0139-C100100 µgCHF 430.00
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Product Details
Synonyms Caspase-11 (mouse); Protease ICH-3 (mouse)
Product Type Monoclonal Antibody
Properties
Clone 8A5
Isotype Rat IgG1κ
Immunogen/Antigen Recombinant mouse caspase-11 (p20 fragment).
Application

ELISA: (recombinant protein)
Flow Cytometry: (overexpressed in cell lines)
Immunoprecipitation: (1-5μg/ml)
Western Blot: (1-2μg/ml)
Optimal conditions must be determined individually for each application.

Crossreactivity Mouse
Rat
Specificity

Recognizes mouse and rat full-length caspase-11 protein. Detects two bands of ~38kDa and ~43kDa by Western blot.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified (sepharose column).
Endotoxin Content <0.1EU/μg purified protein (LAL test; Lonza).
Concentration 1mg/ml
Formulation Liquid. In PBS containing 0.02% sodium azide.
Isotype Negative Control

Rat IgG1 Isotype Control

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Caspase-11 not only activates caspase-1, that is required for the maturation of proinflammatory cytokines such as interleukin-1 (IL-1) and interleukin-18 (IL-18), but also activates caspase-3, leading to cellular apoptosis under pathological conditions. In most cells, caspase-11 is only expressed upon induction with pro-inflammatory stimuli. Caspase-11 is an essential mediator of endotoxic shock and caspase-11-deficient mice are resistant to endotoxic shock. LPS-induced caspase-11 regulates lymphocyte apoptosis by activating both caspase-3 and caspase-7.

Product References
  1. Guanylate-binding protein 5 licenses caspase11 for Gasdermin-D mediated host resistance to Brucella abortus infection: D.M. Cerqueira, et al.; PLoS Pathogens 14, e1007519 (2018)
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