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AdipoGen Life Sciences
anti-Caspase-11 (mouse), mAb (8A5)
Product Details | |
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Synonyms | Caspase-11 (mouse); Protease ICH-3 (mouse) |
Product Type | Monoclonal Antibody |
Properties | |
Clone | 8A5 |
Isotype | Rat IgG1κ |
Immunogen/Antigen | Recombinant mouse caspase-11 (p20 fragment). |
Application |
ELISA: (recombinant protein) |
Crossreactivity |
Mouse Rat |
Specificity |
Recognizes mouse and rat full-length caspase-11 protein. Detects two bands of ~38kDa and ~43kDa by Western blot. |
Purity | ≥95% (SDS-PAGE) |
Purity Detail | Protein G-affinity purified (sepharose column). |
Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
Concentration | 1mg/ml |
Formulation | Liquid. In PBS containing 0.02% sodium azide. |
Isotype Negative Control | |
Shipping and Handling | |
Shipping | BLUE ICE |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
Documents | |
MSDS | Download PDF |
Product Specification Sheet | |
Datasheet | Download PDF |
Caspase-11 not only activates caspase-1, that is required for the maturation of proinflammatory cytokines such as interleukin-1 (IL-1) and interleukin-18 (IL-18), but also activates caspase-3, leading to cellular apoptosis under pathological conditions. In most cells, caspase-11 is only expressed upon induction with pro-inflammatory stimuli. Caspase-11 is an essential mediator of endotoxic shock and caspase-11-deficient mice are resistant to endotoxic shock. LPS-induced caspase-11 regulates lymphocyte apoptosis by activating both caspase-3 and caspase-7.
- Guanylate-binding protein 5 licenses caspase11 for Gasdermin-D mediated host resistance to Brucella abortus infection: D.M. Cerqueira, et al.; PLoS Pathogens 14, e1007519 (2018)