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AdipoGen Life Sciences
anti-Caspase-11 (mouse), mAb (4E11)
430
CHF
CHF 430.00
In stock
AG-20T-0140-C100100 µgCHF 430.00
Figure 1: Western blot using anti-Caspase-11 (mouse), mAb (4E11) (Prod. No. AG-20T-0140) detecting endogenous caspase-11 in mouse spleen and lymph node as two bands of 43 and 38 kDa after exposure to LPS.
Figure 2: Immunoprecipitation of caspase-11 using anti-caspase-11 mAbs (4E11 and 8A5) (Prod. No. AG-20T-0139 and AG-20T-0140).
Figure 3: Flow cytometry data of overexpressed FLAG-tagged caspase-11 in 293T cells using anti-caspase-11 mAbs (4E11 and 8A5) (Prod. No. AG-20T-0139 and AG-20T-0140), anti-FLAG or control.
| Product Details | |
|---|---|
| Synonyms | Caspase-11 (mouse); Protease ICH-3 (mouse) |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | 4E11 |
| Isotype | Rat IgG1κ |
| Immunogen/Antigen | Recombinant mouse caspase-11 (p20 fragment). |
| Application |
ELISA: (recombinant protein) |
| Crossreactivity |
Mouse Rat |
| Specificity |
Recognizes mouse and rat full-length caspase-11 protein. Detects two bands of ~38kDa and ~43kDa by Western blot. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified (sepharose column). |
| Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 0.02% sodium azide. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Caspase-11 not only activates caspase-1, that is required for the maturation of proinflammatory cytokines such as interleukin-1 (IL-1) and interleukin-18 (IL-18), but also activates caspase-3, leading to cellular apoptosis under pathological conditions. In most cells, caspase-11 is only expressed upon induction with pro-inflammatory stimuli. Caspase-11 is an essential mediator of endotoxic shock and caspase-11-deficient mice are resistant to endotoxic shock. LPS-induced caspase-11 regulates lymphocyte apoptosis by activating both caspase-3 and caspase-7.
Product References
- Apaf-1 and caspase-9 are required for cytokine withdrawal-induced apoptosis of mast cells but dispensable for their functional and clonogenic death: V.S. Marsden, et al.; Blood 107, 1872 (2006)
- Apoptosis and non-inflammatory phagocytosis can be induced by mitochondrial damage without caspases: M.F. Van Delft, et al.; Cell Death Differ. 17, 821 (2010)
- Characterisation of mice lacking the inflammatory caspases-1/11/12 reveals no contribution of caspase-12 to cell death and sepsis: R. Salvamoser, et al.; Cell Death Differ. 26, 1124 (2019)
- Flexible Usage and Interconnectivity of Diverse Cell Death Pathways Protect against Intracellular Infection: M. Doerflinger, et al.; Immunity 53, 533 (2020)
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