anti-Adiponectin (mouse), pAb
AG-25A-0004-C100100 µgCHF 390.00
Western blot analysis in mouse plasma using anti-Adiponectin (mouse), pAb (Prod. No. AG-25A-0004) at 1:5'000 dilution.
|Synonyms||ACRP30; AdipoQ; apM1; GBP28; Adipocyte Complement Related Protein of 30kDa|
|Product Type||Polyclonal Antibody|
|Immunogen/Antigen||Recombinant mouse adiponectin.|
ELISA: (direct and indirect; 1:2’000-1:10’000)
Immunoprecipitation: (1-5 μl per sample in a final reaction volume of 0.5-1 ml)
Western Blot: (1:2’000-1:10’000 using ECL; suggested blocking and dilution buffer is PBST with 0.05% Tween 20 with 5% skim milk; incubate 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
|Specificity||Recognizes mouse adiponectin. Weakly cross-reacts with rat adiponectin. Detects a band of ~35kDa by Western blot.|
|Purity Detail||Protein A-affinity purified.|
|Formulation||Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
|Use/Stability||Stable for at least 6 months after receipt when stored at -20°C.|
|Product Specification Sheet|
ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.
- eNOS plays a major role in adiponectin synthesis in adipocytes: E.H. Koh, et al.; Am. J. Physiol. Endocrinol. Metab. 298, E846 (2010)