anti-Adiponectin (mouse), pAb

CHF 390.00
In stock
AG-25A-0004-C100100 µgCHF 390.00
 
More Information
Product Details
Synonyms ACRP30; AdipoQ; apM1; GBP28; Adipocyte Complement Related Protein of 30kDa
Product Type Polyclonal Antibody
Properties
Source/Host Rabbit
Immunogen/Antigen Recombinant mouse adiponectin.
Application ELISA: (direct and indirect; 1:2’000-1:10’000)
Immunoprecipitation: (1-5 μl per sample in a final reaction volume of 0.5-1 ml)
Western Blot: (1:2’000-1:10’000 using ECL; suggested blocking and dilution buffer is PBST with 0.05% Tween 20 with 5% skim milk; incubate 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
Crossreactivity Mouse
Specificity Recognizes mouse adiponectin. Weakly cross-reacts with rat adiponectin. Detects a band of ~35kDa by Western blot.
Purity Detail Protein A-affinity purified.
Concentration 1mg/ml
Formulation Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.
Product References
  1. eNOS plays a major role in adiponectin synthesis in adipocytes: E.H. Koh, et al.; Am. J. Physiol. Endocrinol. Metab. 298, E846 (2010)
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