anti-Adiponectin (mouse), pAb

ELISA: (direct and indirect; 1:2’000-1:10’000)
Immunoprecipitation: (1-5 μl per sample in a final reaction volume of 0.5-1 ml)
Western Blot: (1:2’000-1:10’000 using ECL; suggested blocking and dilution buffer is PBST with 0.05% Tween 20 with 5% skim milk; incubate 1 hour at room temperature).
Optimal conditions must be determined individually for each application.

Recognizes mouse adiponectin. Weakly cross-reacts with rat adiponectin. Detects a band of ~35kDa by Western blot.

Rabbit IgG