AdipoGen Life Sciences

Fc (mouse) IgG2a Control (rec.) (non-lytic)

CHF 340.00
In stock
AG-35B-0017-C01010 µgCHF 340.00
AG-35B-0017-30103 x 10 µgCHF 680.00
AG-35B-0017-C100100 µgCHF 1'360.00
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Product Details
Synonyms Fc Immunoglobulin 2a Fragment Constant
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence

Fc (mouse) IgG2a (100-330 including 4 mutations).

Crossreactivity Mouse
Application

Negative Control

MW ~28kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/μg purified protein (LAL test).
Concentration After reconstitution:
for 10µg size: 0.1mg/ml
for 100µg size: 1mg/ml
Reconstitution Reconstitute with 100µl endotoxin-free water.
Formulation Lyophilized. Contains PBS.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Negative control in experiments with recombinant proteins fused to Fc (mouse IgG2a (non-lytic) (e.g. Prod. No. AG-40B-0231). This protein is not an appropriate negative control for other standard Fc protein constructs. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the non-lytic proteins incapable of antibody-directed cytotoxicity (ADCC) and complement-directed cytotoxicity (CDC).

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