AdipoGen Life Sciences

Fc (mouse) IgG2a Control (rec.) (non-lytic)

CHF 340.00

In stock

AG-35B-0017-C01010 µgCHF 340.00

AG-35B-0017-30103 x 10 µgCHF 680.00

AG-35B-0017-C100100 µgCHF 1'360.00

More Information
Product Details
Synonyms	Fc Immunoglobulin 2a Fragment Constant
Product Type	Protein
Properties
Source/Host	HEK 293 cells
Sequence	Fc (mouse) IgG2a (100-330 including 4 mutations).
Crossreactivity	Mouse
Application	Negative Control
MW	~28kDa (SDS-PAGE)
Purity	≥95% (SDS-PAGE)
Endotoxin Content	<0.01EU/μg purified protein (LAL test).
Concentration	After reconstitution: for 10µg size: 0.1mg/ml for 100µg size: 1mg/ml
Reconstitution	Reconstitute with 100µl endotoxin-free water.
Formulation	Lyophilized. Contains PBS.
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Negative control in experiments with recombinant proteins fused to Fc (mouse IgG2a (non-lytic) (e.g. Prod. No. AG-40B-0231). This protein is not an appropriate negative control for other standard Fc protein constructs. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the non-lytic proteins incapable of antibody-directed cytotoxicity (ADCC) and complement-directed cytotoxicity (CDC).

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