Cookie Policy: This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.
AdipoGen Life Sciences
Nampt (Visfatin/PBEF) (mouse) (rec.)
155
CHF
CHF 155.00
In stock
AG-40A-0056Y-C01010 µgCHF 155.00
AG-40A-0056Y-30103 x 10 µgCHF 310.00
Replaces AG-40A-0056
| Product Details | |
|---|---|
| Synonyms | Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase |
| Product Type | Protein |
| Properties | |
| Source/Host | CHO cells |
| Sequence |
Mouse Nampt (visfatin/PBEF) (aa 1-491) is fused at the N-terminus to a FLAG®-tag. |
| Crossreactivity | Mouse |
| MW | ~55kDa (SDS-PAGE) |
| Purity | ≥90% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg purified protein (LAL test; Lonza). |
| Concentration | 0.1mg/ml after reconstitution. |
| Reconstitution | Reconstitute with 100μl sterile water. |
| Formulation | Lyophilized. Contains PBS. |
| Other Product Data |
UniProt link Q99KQ4: Nicotinamide phosphoribosyltransferase (mouse) FLAG is a registered trademark of Sigma-Aldrich Co. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.
Product References
- SIRT1-Mediated eNAMPT Secretion from Adipose Tissue Regulates Hypothalamic NAD+ and Function in Mice: M.J. Yoon, et al.; Cell Metab. 21, 706 (2015)
- Visfatin and resistin in gonadotroph cells: expression, regulation of LH secretion and signalling pathways: V. Maillard, et al.; Reprod. Fertil. Develop. 29, 2479 (2017)
Related Products
![Measurement of NAMPT enzymatic activity was performed as described previously [G.C. Elliott, et al.; Anal Biochem 107, 199 (1980)]. The recombinant Nampt was diluted in assay buffer and 10μl per 50μl reaction mix were applied in the reaction mix (20](https://adipogen.com/pub/media/catalog/product/cache/small_image/152x190/beff4985b56e3afdbeabfc89641a4582/a/g/ag-40a-0018_h-nampt_visfatin_-his-enzymatic-activity.png)