AdipoGen Life Sciences

HpARI (CCP1/2) (rec.) (His)

CHF 440.00
In stock
AG-40B-0201-C05050 µgCHF 440.00
AG-40B-0201-30503 x 50 µgCHF 880.00
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Product Details
Synonyms Heligmosomoides Polygyrus Alarmin Release Inhibitor Truncated (CCP1/2); HpARI Truncation Lacking CCP3
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence

Heligmosomoides polygyrus protein HpARI (aa 17-165) is fused at the C-terminus to a His-tag.

Crossreactivity Human
Mouse
Specificity

Binds to human and mouse IL-33.

Biological Activity

Enhances activity of mouse or human IL-33  in vivo and in vitro.

MW ~28kDa (SDS-PAGE)
Purity ≥90% (SDS-PAGE)
Endotoxin Content <0.01EU/μg purified protein (LAL test).
Concentration After reconstitution: 1mg/ml
Reconstitution Reconstitute with 50μl sterile water.
Accession Number Worm base HPBE_0000813301
Formulation Lyophilized. Contains PBS.
Other Product Data

Worm base HPBE_0000813301: Heligmosomoides polygyrus protein HpARI

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

HpARI is a protein secreted by the mouse parasite Heligmosomoides polygyrus. The mature protein HpARI, containing three predicted Complement Control Protein (CCP)-like modules (also known as Short Consensus Repeats (SCRs) or sushi-domains), suppresses type 2 (allergic) immune responses through interference in the interleukin-33 (IL-33) pathway. During cell damage, HpARI gains access to the nucleus of necrotic cells, where it binds directly to IL-33 and nuclear DNA, preventing secretion and binding of IL-33 to its receptor. A non-natural truncation consisting of the first 2 domains of HpARI (CCP1/2) retains IL-33 and DNA binding capacity. HpARI (CCP1/2) is able to stabilize IL-33, increasing its half-life and amplifying its effects. HpARI (CCP1/2) increases IL-33 activity by protecting it from oxidation and proteolytic degradation. HpARI (CCP1/2) (rec.) (His) is a new type of reagent to study IL-33-mediated pathology in vivo.

Product References
  1. An IL-1 cytokine member, IL-33, induces human basophil activation via its ST2 receptor: M. Suzukawa, et al.; J. Immunol. 181, 5981 (2008)
  2. HpARI protein secreted by a helminth parasite suppresses interleukin-33: M. Osbourne, et al.; Immunity 47, 739 (2017)
  3. A Truncated Form of HpARI Stabilizes IL-33, Amplifying Responses to the Cytokine: C. Chauche, et al.; Front. Immunol., 11, 1363 (2020)
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