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AdipoGen Life Sciences
HpARI (CCP1/2) (rec.) (His)
440
CHF
CHF 440.00
In stock
AG-40B-0201-C05050 µgCHF 440.00
AG-40B-0201-30503 x 50 µgCHF 880.00
Figure 1: Binding of HpARI (CCP1/2) (rec.) (His) (Prod. No. AG-40B-0201) to human and mouse IL-33.
Method: IL-33 (human) (rec.) (untagged) (Prod. No. AG-40B-0038), IL-33 (mouse) (rec.) (untagged) (Prod. No. AG-40B-0041) as well as an unrelated protein as control are coated on an ELISA plate at 1µg/ml. After blocking and washing steps, indicated concentrations of HpARI (CCP1/2) (rec.) (His) is added. Following incubation for 1h at RT, the binding is detected using an anti-His antibody (HRP).
Method: IL-33 (human) (rec.) (untagged) (Prod. No. AG-40B-0038), IL-33 (mouse) (rec.) (untagged) (Prod. No. AG-40B-0041) as well as an unrelated protein as control are coated on an ELISA plate at 1µg/ml. After blocking and washing steps, indicated concentrations of HpARI (CCP1/2) (rec.) (His) is added. Following incubation for 1h at RT, the binding is detected using an anti-His antibody (HRP).
| Product Details | |
|---|---|
| Synonyms | Heligmosomoides Polygyrus Alarmin Release Inhibitor Truncated (CCP1/2); HpARI Truncation Lacking CCP3 |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells |
| Sequence |
Heligmosomoides polygyrus protein HpARI (aa 17-165) is fused at the C-terminus to a His-tag. |
| Crossreactivity |
Human Mouse |
| Specificity |
Binds to human and mouse IL-33. |
| Biological Activity |
Enhances activity of mouse or human IL-33 in vivo and in vitro. |
| MW | ~28kDa (SDS-PAGE) |
| Purity | ≥90% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg purified protein (LAL test). |
| Concentration | After reconstitution: 1mg/ml |
| Reconstitution | Reconstitute with 50μl sterile water. |
| Accession Number | Worm base HPBE_0000813301 |
| Formulation | Lyophilized. Contains PBS. |
| Other Product Data |
Worm base HPBE_0000813301: Heligmosomoides polygyrus protein HpARI |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
HpARI is a protein secreted by the mouse parasite Heligmosomoides polygyrus. The mature protein HpARI, containing three predicted Complement Control Protein (CCP)-like modules (also known as Short Consensus Repeats (SCRs) or sushi-domains), suppresses type 2 (allergic) immune responses through interference in the interleukin-33 (IL-33) pathway. During cell damage, HpARI gains access to the nucleus of necrotic cells, where it binds directly to IL-33 and nuclear DNA, preventing secretion and binding of IL-33 to its receptor. A non-natural truncation consisting of the first 2 domains of HpARI (CCP1/2) retains IL-33 and DNA binding capacity. HpARI (CCP1/2) is able to stabilize IL-33, increasing its half-life and amplifying its effects. HpARI (CCP1/2) increases IL-33 activity by protecting it from oxidation and proteolytic degradation. HpARI (CCP1/2) (rec.) (His) is a new type of reagent to study IL-33-mediated pathology in vivo.
Product References
- An IL-1 cytokine member, IL-33, induces human basophil activation via its ST2 receptor: M. Suzukawa, et al.; J. Immunol. 181, 5981 (2008)
- HpARI protein secreted by a helminth parasite suppresses interleukin-33: M. Osbourne, et al.; Immunity 47, 739 (2017)
- A Truncated Form of HpARI Stabilizes IL-33, Amplifying Responses to the Cytokine: C. Chauche, et al.; Front. Immunol., 11, 1363 (2020)
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