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AdipoGen Life Sciences
IL-12 (mouse):Fc (LALA-PG)-KIH (human) (rec.)
440
CHF
CHF 440.00
In stock
AG-40B-0255-C05050 µgCHF 440.00
Figure 1: Protein structure of IL-12 (mouse):Fc (LALA-PG)-KIH (human) (rec.) (Prod. No. AG-40B-0255) containing a single heterodimer.
| Product Details | |
|---|---|
| Synonyms | InVivoKine™; IL-12 (mouse):Fc (LALA-PG) Knobs-into-Holes (human) (rec.); Interleukin-12 Subunit α:Interleukin-12 Subunit β; CLMF 35kDa Subunit:CLMF 40kDa Subunit; IL-12 Subunit p35:IL-12 Subunit |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells. Produced using animal component-free medium. |
| Sequence |
IL-12A/p35 (aa 23-215) (mouse):Fc (LALA-PG) (human) Knobs and IL-12B/p40 (aa 23-335) (mouse):Fc (LALA-PG) (human) Holes form the IL-12 (mouse):Fc (LALA-PG)-KIH (human) using the Knobs-into-Holes technology (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)). |
| Crossreactivity | Mouse |
| Specificity |
Binds to the mouse IL-12Rβ1 protein. Fc (LALA-PG)-KIH (human) does not bind to FcRs. |
| MW | ~60kDa and 75 kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.02EU/μg purified protein (LAL test) |
| Concentration | 1mg/ml after reconstitution. |
| Reconstitution | Reconstitute with 50μl sterile water. |
| Accession Number | P43431, P43432 |
| Formulation | Lyophilized. Contains PBS |
| Protein Negative Control |
Fc (LALA-PG)-KIH (human) IgG1 Control (rec.) (Prod. No. AG-35B-0018) |
| Other Product Data |
UniProt link P43431: IL-12A (mouse) UniProt link P43432: IL-12B (mouse) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Interleukin-12 (IL-12) family members are heterodimer glycoproteins, composed of two covalently linked subunits, α and β chains. The α-subunit consists of IL-23p19, IL-27p28 and IL-12p35, and the β-subunit includes IL-12p40 and Epstein-Barr virus-induced gene (Ebi3). IL-12 members bind to cognate heterodimeric receptor chains expressed on T cells.
This family includes IL-12, IL-23, IL-27, IL-35 and IL-39. IL-12 and IL-23 are predominantly proinflammatory cytokines that contribute key roles in the development of Th1 and Th17 cells, respectively. IL-27 has both pro- and anti-inflammatory properties and is a potent T cell immunomodulator. IL-35, a new member of this family, is a potent inhibitory cytokine produced by natural, thymus-derived regulatory T cell (nTreg) populations. IL-39, the newest member of IL-12 family, mediates the inflammatory response through the activation of STAT1/STAT3 signaling pathway. These IL-12 family members link innate immunity with the development of adaptive immunity and are also important for regulating T cell responses.
IL-12 is composed of two subunits IL-12A/p35 and IL-12B/p40 and signals via a high-affinity IL-12R, leading to activation of STAT 4. IL-12 is produced by macrophages, dendritic cells and B cells. The key role of the pro-inflammatory IL-12 is to induce the IFN-γ which regulates the Th responses, to promote the naïve T cells to directly differentiate into effector cells (Th1) and then release IFN-γ.
The protein IL-12 (mouse):Fc (LALA-PG)-KIH (human) (rec.) is produced by using two different vectors, one encoding for the IL-12A/p35:Fc (LALA-PG) Knobs sequence (synthesizing a protein of 60kDa) and one encoding for the IL12B/p40:Fc (LALA-PG) Holes sequence (synthesizing a protein of 75kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization and for secretion of the final protein IL-12 (mouse):Fc (LALA-PG)-KIH (human) (rec.). The LALA-PG mutations inhibit binding to FcγRs and C1q while FcRn binding and Fc stability remain unaffected.
InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research, developed and manufactured by AdipoGen Life Sciences.
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