AdipoGen Life Sciences

IL-12 (mouse):Fc-KIH (human) (rec.)

CHF 440.00
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AG-40B-0240-C05050 µgCHF 440.00
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Product Details
Synonyms IL-12 (mouse):Fc Knobs-into-Holes (human) (rec.); Interleukin-12 Subunit α:Interleukin-12 Subunit β; CLMF 35kDa Subunit:CLMF 40kDa Subunit; IL-12 Subunit p35:IL-12 Subunit
Product Type Protein
Source/Host CHO cells. Produced using animal component-free medium.

IL-12A/p35 (aa 23-215) (mouse):Fc Knobs and IL-12B/p40 (aa 23-335) (mouse):Fc Holes form the IL-12 (mouse):Fc-KIH (human) using the Knobs-into-Holes technology (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)).

Crossreactivity Mouse

Binds to the mouse IL-12Rβ1 protein. Fc (LALA-PG)-KIH (human) does not bind to FcRs.

MW ~60kDa and 75 kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/μg purified protein (LAL test).
Concentration 1mg/ml after reconstitution.
Reconstitution Reconstitute with 50μl sterile water.
Accession Number P43431, P43432
Formulation Lyophilized. Contains PBS
Protein Negative Control

Fc-KIH (human) IgG1 Control (rec.) (Prod. No. AG-35B-0015)

Other Product Data

UniProt link P43431:  IL-12A (mouse)

UniProt link P43432: IL-12B (mouse)

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
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Product Specification Sheet
Datasheet Download PDF

Interleukin-12 (IL-12) family members are heterodimer glycoproteins, composed of two covalently linked subunits, α and β chains. The α-subunit consists of IL-23p19, IL-27p28 and IL-12p35, and the β-subunit includes IL-12p40 and Epstein-Barr virus-induced gene (Ebi3). IL-12 members bind to cognate heterodimeric receptor chains expressed on T cells.

This family includes IL-12, IL-23, IL-27, IL-35 and IL-39. IL-12 and IL-23 are predominantly proinflammatory cytokines that contribute key roles in the development of Th1 and Th17 cells, respectively. IL-27 has both pro- and anti-inflammatory properties and is a potent T cell immunomodulator. IL-35, a new member of this family, is a potent inhibitory cytokine produced by natural, thymus-derived regulatory T cell (nTreg) populations. IL-39, the newest member of IL-12 family, mediates the inflammatory response through the activation of STAT1/STAT3 signaling pathway. These IL-12 family members link innate immunity with the development of adaptive immunity and are also important for regulating T cell responses.

IL-12 is composed of two subunits IL-12A/p35 and IL-12B/p40 and signals via a high-affinity IL-12R, leading to activation of STAT 4. IL-12 is produced by macrophages, dendritic cells and B cells. The key role of the pro-inflammatory IL-12 is to induce the IFN-γ which regulates the Th responses, to promote the naïve T cells to directly differentiate into effector cells (Th1) and then release IFN-γ. 

The protein IL-12 (mouse):Fc-KIH (human) (rec.) is produced by using two different vectors, one encoding for the IL-12A/p35:Fc Knobs sequence (synthesizing a protein of 60kDa) and one encoding for the IL12B/p40:Fc Holes sequence (synthesizing a protein of 75kDa). Both vectors transfected into CHO cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization and for secretion of the final protein IL-12 (mouse):Fc-KIH (human) (rec.).

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