KillerTRAIL™, Soluble (human) (rec.)

CHF 470.00
In stock
AG-40T-0001-C05050 µgCHF 470.00
AG-40T-0001-30503 x 50 µgCHF 940.00
AG-40T-0001-C500500 µgCHF 1'700.00
More Information
Product Details
Synonyms TRAIL; Apo-2L; TNFSF10; CD253
Product Type Protein
Source/Host E. coli

The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide.

Crossreactivity Human

Binds to human and mouse TRAIL receptors and osteoprotegerin (OPG).

Biological Activity

Potent biologically active trimer. Induces apoptosis in a concentration range of 10-100ng/ml. Note: Does not require a cross-linking enhancer for its potent biological activity. ED50: 20ng/ml (BJAB cells). For cell lines that require extensive cross-linking of the TRAIL-Rs for killing (e.g. Jurkat) use SuperKillerTRAIL™ (Prod. No. AG-40T-0002).

MW ~24kDa (SDS-PAGE)
Purity ≥98% (SDS-PAGE)
Purity Detail Affinity purified. Sterile filtered.
Endotoxin Content ≤0.01EU/μg purified protein (LAL test; Lonza).
Concentration 50µg Vials: 0.5 mg/ml
500µg Vials: Lot dependent
Formulation Liquid. In 20mM HEPES, pH 7.4, 150mM NaCl, 0.005% Tween 20, 1% sucrose, 1mM DTT.
Other Product Data

UniProt link P50591: TRAIL (human) | Note: To retain full activity the concentration of the aliquots should be at least 0.1mg/ml.

Shipping and Handling
Shipping DRY ICE
Short Term Storage -20°C
Long Term Storage -80°C
Handling Advice After opening, prepare aliquots and store at -80°C.
Avoid freeze/thaw cycles.
For maximum product recovery after thawing, centrifuge the vial before opening the cap.
Use/Stability Stable for at least 1 year after receipt when stored at -80°C.
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

TNF-related apoptosis-inducing ligand (TRAIL; Apo2L;CD253; TNFSF10) is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-R1 (DR4; Apo2;CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A;TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1;LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2;TRUNDD; CD264; TNFRSF10D), and osteoprotegerin (OPG; OCIF; TNFRSF11B). Trimerized TRAIL triggers apoptosis upon ligation of cell surface TRAIL-R1 and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC).

Product References
  1. Crucial role of reactive oxygen species (ROS) for the proapoptotic effects of indirubin derivative DKP-073 in melanoma cells: V. Zhivkova, et al.; Mol. Carcinog., ahead of print (2018)
© 2017 Adipogen Life Sciences. Pictures: © 2012 Martin Oeggerli. All Rights Reserved.