Cookie Policy: This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.
AdipoGen Life Sciences
KillerTRAIL™, Soluble (human) (rec.)
470
CHF
CHF 470.00
In stock
AG-40T-0001-C05050 µgCHF 470.00
AG-40T-0001-30503 x 50 µgCHF 940.00
AG-40T-0001-C500500 µgCHF 1'900.00
| Product Details | |
|---|---|
| Synonyms | TRAIL; Apo-2L; TNFSF10; CD253 |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Sequence |
The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. |
| Crossreactivity |
Human Mouse |
| Specificity |
Binds to human TRAIL receptors. Binding to OPG has not been tested experimentally. |
| Biological Activity |
Potent biologically active trimer. Induces apoptosis in a concentration range of 10-100ng/ml. Note: Does not require a cross-linking enhancer for its potent biological activity. ED50: 20ng/ml (BJAB cells). For cell lines that require extensive cross-linking of the TRAIL-Rs for killing (e.g. Jurkat) use SuperKillerTRAIL™ (Prod. No. AG-40T-0002). |
| MW | ~24kDa (SDS-PAGE) |
| Purity | ≥98% (SDS-PAGE) |
| Purity Detail | Affinity purified. Sterile filtered. |
| Endotoxin Content | ≤0.01EU/μg purified protein (LAL test; Lonza). |
| Concentration |
50µg Vials: 0.5 mg/ml 500µg Vials: Lot dependent |
| Formulation | Liquid. In 20mM HEPES, pH 7.4, 150mM NaCl, 0.005% Tween 20, 1% sucrose, 1mM DTT. |
| Other Product Data |
UniProt link P50591: TRAIL (human) | Note: To retain full activity the concentration of the aliquots should be at least 0.1mg/ml. |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -80°C |
| Handling Advice |
After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -80°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
TNF-related apoptosis-inducing ligand (TRAIL; Apo2L;CD253; TNFSF10) is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-R1 (DR4; Apo2;CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A;TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1;LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2;TRUNDD; CD264; TNFRSF10D), and osteoprotegerin (OPG; OCIF; TNFRSF11B). Trimerized TRAIL triggers apoptosis upon ligation of cell surface TRAIL-R1 and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC).
Product References
- Functional TRAIL receptors in monocytes and tumor-associated macrophages: A possible targeting pathway in the tumor microenvironment: M. Liguori, et al.; Oncotarget 7, 41662 (2016)
- Crucial role of reactive oxygen species (ROS) for the proapoptotic effects of indirubin derivative DKP-073 in melanoma cells: V. Zhivkova, et al.; Mol. Carcinog. 58, 258 (2019)
- Mcl‐1 targeting strategies unlock the proapoptotic potential of TRAIL in melanoma cells: Z. Sarif, et al.; Mol. Carcinog. 59, 1256 (2020)
- Crucial Role of Reactive Oxygen Species (ROS) for the Proapoptotic Effects of Indirubin Derivatives in Cutaneous SCC Cells: J. Zhu, et al.; Antioxidants 10, 1514 (2021)
- High ROS Production by Celecoxib and Enhanced Sensitivity for Death Ligand-Induced Apoptosis in Cutaneous SCC Cell Lines: J. Zhu, et al.; Int. J. Mol. Sci. 22, 3622 (2021)
- Silencing of Mcl-1 overcomes resistance of melanoma cells against TRAIL-armed oncolytic adenovirus by enhancement of apoptosis: B. Tolksdorf, et al.; J. Mol. Med. 99, 1279 (2021)
Related Products
