SuperKillerTRAIL™, Soluble (human) (rec.)
|Synonyms||TRAIL; Apo-2L; TNFSF10; CD253|
|Sequence||The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.|
|Specificity||Binds to human TRAIL receptors.|
|Biological Activity||Induces apoptosis at concentrations of >1ng/ml. Note: Does not require a cross-linking enhancer for its potent biological activity. Shows superior activity on human cell lines that require extensive cross-linking of TRAIL-Rs for killing (e.g. Jurkat).|
|Purity Detail||Affinity purified. Sterile filtered.|
|Endotoxin Content||<0.01EU/μg purified protein (LAL test; Lonza).|
|Formulation||Liquid. In 20mM HEPES, pH 7.4, 150mM NaCl, 0.005% Tween 20, 1% sucrose, 1mM DTT.|
|Other Product Data||
In order to retain full activity do not dilute higher than to a concentration of 0.1mg/ml, prepare 20µl aliquots and store at -80°C. Working dilution: 2-100ng/ml.|
UniProt link P50591: Shipping and Handling
|Short Term Storage||-20°C|
|Long Term Storage||-80°C|
|Handling Advice||Avoid freeze/thaw cycles.|
Once thawed it is recommended to prepare appropriate aliquots and to store them at -80°C.
Stable for at least 1 year after receipt when stored at -80°C.
|Product Specification Sheet|
TNF-related apoptosis-inducing ligand (TRAIL; Apo2L;CD253; TNFSF10) is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-R1 (DR4; Apo2;CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A;TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1;LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2; TRUNDD; CD264; TNFRSF10D), and osteoprotegerin (OPG; OCIF; TNFRSF11B). Trimerized TRAIL triggers apoptosis upon ligation of cell surface TRAIL-R1 and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC).
- Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory “FADDosome” Complex upon TRAIL Stimulation: C.M. Henry & S.J. Martin; Mol. Cell 65, 715 (2017)