SuperKillerTRAIL™, Soluble (human) (rec.)

CHF 400.00
In stock
AG-40T-0002-C02020 µgCHF 400.00
AG-40T-0002-30203 x 20 µgCHF 800.00
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Product Details
Synonyms TRAIL; Apo-2L; TNFSF10; CD253
Product Type Protein
Source/Host E. coli

The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.

Crossreactivity Human

Binds to human TRAIL receptors.

Biological Activity

Induces apoptosis at concentrations of >1ng/ml. Note: Does not require a cross-linking enhancer for its potent biological activity. Shows superior activity on human cell lines that require extensive cross-linking of TRAIL-Rs for killing (e.g. Jurkat).

Purity ≥98% (SDS-PAGE)
Purity Detail Affinity purified. Sterile filtered.
Endotoxin Content <0.01EU/μg purified protein (LAL test; Lonza).
Concentration 0.5mg/ml
Formulation Liquid. In 20mM HEPES, pH 7.4, 150mM NaCl, 0.005% Tween 20, 1% sucrose, 1mM DTT.
Other Product Data

In order to retain full activity do not dilute higher than to a concentration of 0.1mg/ml, prepare 20µl aliquots and store at -80°C. Working dilution: 2-100ng/ml.

UniProt link P50591: TRAIL (human)

Shipping and Handling
Shipping DRY ICE
Short Term Storage -20°C
Long Term Storage -80°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability Once thawed it is recommended to prepare appropriate aliquots and to store them at -80°C.
Stable for at least 1 year after receipt when stored at -80°C.
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

TNF-related apoptosis-inducing ligand (TRAIL; Apo2L;CD253; TNFSF10) is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-R1 (DR4; Apo2;CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A;TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1;LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2; TRUNDD; CD264; TNFRSF10D), and osteoprotegerin (OPG; OCIF; TNFRSF11B). Trimerized TRAIL triggers apoptosis upon ligation of cell surface TRAIL-R1 and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC).

Product References
  1. Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory “FADDosome” Complex upon TRAIL Stimulation: C.M. Henry & S.J. Martin; Mol. Cell 65, 715 (2017)
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