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AdipoGen Life Sciences
TRAIL, Soluble (human) (rec.)
85
CHF
CHF 85.00
In stock
AG-40B-0003-C01010 µgCHF 85.00
AG-40B-0003-50105 x 10 µgCHF 250.00
| Product Details | |
|---|---|
| Synonyms | Apo-2L; TNFSF10; CD253 |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Sequence | The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a FLAG®-tag. |
| Crossreactivity |
Human Mouse |
| Biological Activity | Induces apoptosis of human Jurkat T cells in a concentration range of 5-50ng/ml in the presence of 1μg/ml TNF Ligands Enhancer (Prod. No. AG-35B-0001). In the absence of TNF Ligands Enhancer, TRAIL, Soluble (human) (recombinant) is poorly active. |
| MW | ~23kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg purified protein (LAL test; Lonza). |
| Concentration | 0.1mg/ml after reconstitution. |
| Reconstitution | Reconstitute with 100μl sterile water. |
| Formulation | Lyophilized. Contains PBS. |
| Other Product Data |
UniProt link P50591: TRAIL (human) AfCS Signalling Gateway link A002862: TRAIL (mouse) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
TNF-related apoptosis-inducing ligand (TRAIL; Apo2L;CD253; TNFSF10) is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. Active TRAIL forms trimers and specifically binds to fi ve distinct receptors: TRAIL-R1 (DR4; Apo2;CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A;TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1;LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2;TRUNDD; CD264; TNFRSF10D), and osteoprotegerin (OPG; OCIF; TNFRSF11B). Trimerized TRAIL triggers apoptosis upon ligation of cell surface TRAIL-R1 and/or TRAIL-R2 by inducingthe formation of the so-called multiprotein death-inducing signaling complex (DISC).
Product References
- TNF-Related Apoptosis-Inducing Ligand (TRAIL) - Armed Exosomes Deliver Proapoptotic Signals to Tumor Site: L. Rivoltini, et al.; Clin. Cancer Res. 22, 3499 (2016)
- Hypertonicity-enforced BCL-2 addiction unleashes the cytotoxic potential of death receptors: S. Sirtl, et al.; Oncogene 37, 4122 (2018)
- Tumor necrosis factor receptor family costimulation increases regulatory T-cell activation and function via NF-κB: M. Lubrano di Ricco, et al.; Eur. J. Immunol. 50, 972 (2020)
- Silencing of Mcl-1 overcomes resistance of melanoma cells against TRAIL-armed oncolytic adenovirus by enhancement of apoptosis: B. Tolksdorf, et al.; J. Mol. Med. 99, 1279 (2021)
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