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AdipoGen Life Sciences
SuperKillerTRAIL™, Soluble (mouse) (rec.)
420
CHF
CHF 420.00
In stock
AG-40T-0004-C02020 µgCHF 420.00
AG-40T-0004-30203 x 20 µgCHF 990.00
Figure 1: SuperKillerTRAIL™, Soluble (mouse) (rec.) (Prod. AG-40T-0004) induces apoptosis.
Method: Indicated concentrations of SuperKillerTRAIL™, Soluble (mouse) (rec.) were added to human BJAB target cells (50’000 cells in 100µl RPMI medium containing 10% fetal calf serum) and incubated over night at 37°C in a 96-well plate. Cell viability was determined using PMS/MTS-based cell proliferation assay test. Percentage of apoptosis inhibition was calculated based on the OD measured at 490nm.
Method: Indicated concentrations of SuperKillerTRAIL™, Soluble (mouse) (rec.) were added to human BJAB target cells (50’000 cells in 100µl RPMI medium containing 10% fetal calf serum) and incubated over night at 37°C in a 96-well plate. Cell viability was determined using PMS/MTS-based cell proliferation assay test. Percentage of apoptosis inhibition was calculated based on the OD measured at 490nm.
| Product Details | |
|---|---|
| Synonyms | TRAIL; Apo-2L; TNFSF10; CD253 |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Sequence |
The extracellular domain of mouse TRAIL (aa 99-291) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge. |
| Crossreactivity |
Human Mouse |
| Specificity |
Binds to mouse and less potently to human TRAIL receptors. |
| Biological Activity |
Induces apoptosis at concentrations of >10ng/ml as tested on human tumor cells. This effect can be efficiently blocked by recombinant mouse TRAIL receptors. This protein does not require a cross-linker (enhancer) for its potent biological activity. |
| MW | ~24kDa |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Affinity purified. Sterile filtered. |
| Endotoxin Content | <0.01EU/μg purified protein (LAL test; Lonza). |
| Concentration | 0.5mg/ml |
| Formulation | Liquid. In 20mM HEPES, pH 7.4, 300mM NaCl, 0.01% Tween 20, 1% sucrose, 1mM DTT. |
| Other Product Data |
Note: To retain full activity the concentration of the aliquots should be at least 0.1mg/ml. UniProt link P50592: TRAIL (mouse) |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -80°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability |
Once thawed it is recommended to prepare appropriate aliquots and to store them at -80°C. Stable for at least 1 year after receipt when stored at -80°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
TNF-related apoptosis-inducing ligand (TRAIL; Apo2L;CD253; TNFSF10) is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-R1 (DR4; Apo2;CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A;TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1;LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2; TRUNDD; CD264; TNFRSF10D), and osteoprotegerin (OPG; OCIF; TNFRSF11B). Trimerized TRAIL triggers apoptosis upon ligation of cell surface TRAIL-R1 and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC).
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