anti-CD133 (human), mAb (ANC9C5) (FITC)

Figure 1: Antibody ANC9C5 was tested for its ability to bind to full length CD133 transfectants Y-79 retinoblastoma cells, and other CD133+ cells in flow cytometry. Five x 10^5 human Y-79 retinoblastoma cells per tube were pre-incubated with 20 µl of 250 µg/ml human Ig (to block non-specific binding) after which they were incubated 45 min on ice with 80 µl of anti-CD133/FITC (#ANC-363-040) at a dilution factor of 1:50 (15 µg/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.45 log10 fluorescent units when compared to Mouse IgG1/FITC Isotype control (#ANC-278-040). Binding was blocked when cells were pre incubated 10 min with 20 µl of 0.5 mg/ml of unconjugated anti-CD133 antibody (#ANC-363-020).