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Ancell
anti-CD133 (human), mAb (ANC9C5) (preservative free)
306
CHF
CHF 306.00
In stock
ANC-363-820100 µgCHF 306.00
Figure 1: Antibody ANC9C5 was tested for its ability to bind to full length CD133 transfectants Y-79 retinoblastoma cells, and other CD133+ cells in flow cytometry. Five x 10^5 human Y-79 retinoblastoma cells were washed incubated 45 min on ice with 80 µl of anti-human CD133 antibody (#ANC-363-820) at 5 µg/ml. Cells were washed twice and incubated with Goat-anti-Mouse/FITC, after which they were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.9 log10 fluorescent units when compared to isotype control Mouse IgG1 antibody (#ANC-278-010).
| Product Details | |
|---|---|
| Synonyms | Prominin-1; PROM1; AC133; Prominin-like Protein 1 |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | ANC9C5 |
| Isotype | Mouse IgG1κ |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | WERI-Rb-1 human retinoblastoma cells. |
| Label/Conjugates | Preservative Free |
| Application |
Flow Cytometry |
| Crossreactivity | Human |
| Specificity |
Recognizes epitope 1 of human CD133. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein A purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid, 10 mM Sodium Phosphate pH 7.5, 20 mM Potassium Chloride, 130mM NaCl. |
| Isotype Negative Control | |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| Declaration | Manufactured by Ancell Corporation. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice |
Avoid freeze/thaw cycles. Keep sterile. |
| Use/Stability | Stable for 6 months at 4℃ from date of shipment. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Human CD133 is a 117kd pentaspan transmembrane glycoprotein expressed by a subset of hematopoetic and other types of stem cells found in blood and a variety of tissues. Increased expression of CD133 may be a predictor of decreased prognosis in patients with metastatic cancer. Antibody ANC9C5 recognizes epitope 1 of human CD133 (AC133) present on full length CD133 transfectants, Y79 retinoblastoma and other cell types. Antibody ANC9C5 has been used to image cancer stem cells in a colon carcinoma xenograft.
Product References
- Basic studies on radioimmunotargeting of CD133-positive HCT116 cancer stem cells: Z.-H. Jin, et al.; Mol. Imaging 11, 445 (2012)
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