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BellBrook
Hydriscreener™ NADPH Assay Kit
CHF 0.00
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Figure 1: Schematic overview of the Hydriscreener™ NADPH Assay.
Specifications / Handling
| Product Details | |
|---|---|
| Synonyms | Reduced Nicotinamide Adenine Dinucleotide Phosphate |
| Product Type | Kit |
| Properties | |
| Application Set | Compound Screening |
| Specificity |
NADPH |
| Crossreactivity | Human |
| Quantity |
1000 assays/384wells (BBL-3057-1K) 10000 assays/384wells (BBL-3057-10K) Note: The exact number of assays depends on enzyme reaction conditions. It is important to use assay plates that are entirely black with a nonbinding surface. We recommend Corning® 384-well plates (Cat.# 4514) (BBL-2229 and BBL-2230). The suggested plate has a square well top that enables easier robotic pipetting and a round bottom that allows good Z’ factors. It has a recommended working volume of 15-20 µL. |
| Detection Type | Fluorescent |
| Kit Contains |
- Converting Enzyme |
| Other Product Data |
Click here for Complete Information from the Original Manufacturer |
| Declaration | Manufactured by BellBrook Labs. |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Documents | |
| Manual |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Scientific Background Information
Product Description
The Hydriscreener™ NADPH Assay is a homogeneous, HTS-compatible method to measure NADPH production or consumption by dehydrogenase enzymes. The assay utilizes a fluorogenic substrate, HydriFluor™, that is reduced by NADPH to form a red fluorescent product in the presence of a Converting Enzyme. The Hydriscreener assay has been optimized and validated for high throughput screening (HTS) and inhibitor dose response measurements using multimode plate readers.
The assay is designed to be performed in endpoint mode. Following incubation of the Dehydrogenase enzyme reaction for the desired period, the detection reagents are added along with a quenching reagent (e.g., EDTA) to stop the dehydrogenase reaction. The reduced HydriFluor (HydriFluorRED) can be read on any instrument capable of reading red fluorescence (590 nm). Please note that reductants like DTT and TCEP are not recommended for use with the assay as they cause interference at low micromolar
concentrations.
This assay is designed to be used with purified enzyme preparations. Hydriscreener assays are not validated with cell lysates, blood, serum, or other biological samples.
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