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  1. 20S Constitutive Proteasome Assay Kit
    SBB-KP0038
    SBB-KP0038-KI011 Kit
    CHF 625.00
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    20S Immunoproteasome Activity Raw Data Output: Several wells of Immunoproteasome shown digesting LLVY, PAL, and ANW-AMC over time +/- 1x (40μM) inhibitor (ONX-0914).
  2. 20S Immunoproteasome Assay Kit
    SBB-KP0037
    SBB-KP0037-KI011 Kit
    CHF 772.00
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    % Signal to Background of Continuous Real-Time TR-FRET Parkin titration (autoubiquitination): Serial dilutions of Parkin W403A from 50nM to 3.125nM and 300nM wt Parkin were mixed with UBA1, UBE2D3 and trf-Ub (pS65) mix. Reactions were initiated wit
  3. Cullin4a/Rbx1/DDB1/CRBN E3 Ligase Complex TR-FRET Kit
    SBB-KF0120
    SBB-KF0120-KI011 Kit
    CHF 1'250.00
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    Cullin4a/Rbx1/DDB1/CRBN E3 Ligase Complex TR-FRET Kit
  4. Nedd4 E3 Ligase TR-FRET Kit
    SBB-KF0056
    SBB-KF0056-KI011 Kit
    CHF 1'250.00
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    % Signal to Background of Continuous Real-Time TR-FRET Parkin titration (autoubiquitination): Serial dilutions of Parkin W403A from 50nM to 3.125nM and 300nM wt Parkin were mixed with UBA1, UBE2D3 and trf-Ub (pS65) mix. Reactions were initiated wit
  5. Parkin E3 Ligase TR-FRET Kit
    SBB-KF0036
    SBB-KF0036-KI011 Kit
    CHF 1'250.00
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    Parkin E3 Ligase TR-FRET Kit
  6. SUMO1 (human) (rec.) (Rhodamine 110)
    SBB-PS0028
    SBB-PS0028-C05050 µg
    CHF 434.00
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    Signal to Background: The signal to background ratio was determined by 100% hydrolysis of 200nM, 100nM, 50nM SUMO1-Rhodamine 110 to liberate the quenched conjugate. Assay Buffer: 50mM HEPES pH 7.5, 1mM TCEP, 0.1mg/ml BSA.
  7. SUMO2 (human) (rec.) (Rhodamine 110)
    SBB-PS0029
    SBB-PS0029-C05050 µg
    CHF 434.00
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    Signal to Background: The signal to background ratio was determined by 100% hydrolysis of 200nM, 100nM, 50nM SUMO2-Rhodamine 110 to liberate the quenched conjugate. Assay Buffer: 50mM HEPES pH 7.5, 1mM TCEP, 0.1mg/ml BSA.
  8. UcH-L3 (human) (rec.) (untagged)
    SBB-DE0023
    SBB-DE0023-C05050 µg
    CHF 278.00
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    Michaelis–Menten Kinetics: Ubiquitin Rhodamine 110 serially diluted from 1.6 to 0.05µM was digested with 30pM UcHL3 over time. The assay was carried out in a reaction buffer of 50mM HEPES pH 7.5, 100mM NaCl, 1mM TCEP, 0.1mg/ml BSA, at 25°C. Initial
  9. XIAP E3 Ligase TR-FRET Kit
    SBB-KF0057
    SBB-KF0057-KI011 Kit
    CHF 1'250.00
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    % Signal to Background of Continuous Real-Time TR-FRET NEDD4 titration (autoubiquitination): Serial dilutions of NEDD4 from 50nM to 3.125nM mixed with UBA1, UBE2L3, and, TRF-Ub mix. Reaction was initiated with addition of Mg-ATP.

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