|Synonyms||9-[4-[bis[2-[(Acetyloxy)methoxy]-2-oxoet; Rhod-2 AM|
|Purity Chemicals||≥80% (HPLC)|
|Solubility||Soluble in DMSO, DMF, methanol or chloroform.|
|Declaration||Manufactured by Chemodex.|
|Other Product Data||
Click here for Original Manufacturer Product Datasheet
|Shipping and Handling|
|Short Term Storage||-20°C|
|Long Term Storage||-20°C|
Keep cool and dry.
Protect from light and moisture.
|Use/Stability||Stable for at least 2 years after receipt when stored at -20°C.|
|Product Specification Sheet|
Long-wavelength cell-permeant Ca2+ indicator. Rhod-2/AM is an acetoxymethyl ester derivative of Rhod 2 that can be easily loaded into cells via incubation and used for quantifying intracellular Ca2+ concentration allowing non-invasive measurement of calcium ions in live cells. This fluorogenic probe for intracellular Ca2+ operates in the visible spectrum. Rhod-2/AM itself does not bind Ca2+, but it is readily hydrolyzed to Rhod-2 by endogenous esterases once the dye enters cells. Rhod-2 is a valuable alternative to Fluo-3 to investigate changes in intracellular free Ca2+ concentrations, especially in cells and tissues that have high levels of autofluorescence. Rhod-2 has the longest fluorescent emission signal of the commonly used calcium indicators. It contains a rhodamine-like fluorophore, whose excitation and emission maxima make it suitable for use with argon and krypton laser. Its signal intensity is the strongest of all the calcium probes. Spectral Data: λex 550nm, λem 578nm in methanol; λex 549nm, λem 578nm in DMSO; λex 550nm, λem 570nm in Chloroform:Methanol (90:10).
(1) A. Minta, et al.; J. Biol. Chem. 264, 8171 (1989) | (2) T.M. Quinton, et al.; BBRC 184, 893 (1992) | (3) M. Okada, et al.; Brain Res. 583, 227 (1992) | (4) Y. Tamura, et al.; Brain Res. 592, 317 (1992) | (5) N. Melamed, et al.; J. Neurosci. 13, 632 (1993) | (6) C. Du, et al.; Biophys. J. 80, 549 (2001) | (7) Y.N. Tallini, et al.; PNAS 103, 4753 (2006) | (8) P.R. Territo, et al.; Appl. Spectrosc. 61, 138 (2007) | (9) B.R. Choi; Methods Mol. Biol. 937, 217 (2013)