Chemodex

Rhod 2-AM

CHF 204.00

In stock

CDX-A0072-M0011 mgCHF 204.00

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Product Details
Synonyms	9-[4-[bis[2-[(Acetyloxy)methoxy]-2-oxoet; Rhod-2 AM
Product Type	Chemical
Properties
Formula	C₅₂H₅₉BrN₄O₁₉
MW	1123.96
CAS	145037-81-6
Source/Host Chemicals	Synthetic.
Purity Chemicals	≥80% (HPLC)
Appearance	Red-purple solid.
Solubility	Soluble in DMSO, DMF, methanol or chloroform.
Declaration	Manufactured by Chemodex.
Other Product Data	Click here for Original Manufacturer Product Datasheet Our product description may differ slightly from the original manufacturers product datasheet.
InChi Key	RWXWZOWDWYQKBK-UHFFFAOYSA-M
Smiles	O=C(OCOC(C)=O)CN(CC(OCOC(C)=O)=O)C1=C(C=C(C=C1)C2=C3C=C/C(C=C3OC4=CC(N(C)C)=CC=C24)=[N+](C)/C)OCCOC5=C(C=CC(C)=C5)N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O.[Br-]
Shipping and Handling
Shipping	AMBIENT
Short Term Storage	-20°C
Long Term Storage	-20°C
Handling Advice	Keep cool and dry. Protect from light and moisture.
Use/Stability	Stable for at least 2 years after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Long-wavelength cell-permeant Ca2+ indicator. Rhod-2/AM is an acetoxymethyl ester derivative of Rhod 2 that can be easily loaded into cells via incubation and used for quantifying intracellular Ca2+ concentration allowing non-invasive measurement of calcium ions in live cells. This fluorogenic probe for intracellular Ca2+ operates in the visible spectrum. Rhod-2/AM itself does not bind Ca2+, but it is readily hydrolyzed to Rhod-2 by endogenous esterases once the dye enters cells. Rhod-2 is a valuable alternative to Fluo-3 to investigate changes in intracellular free Ca2+ concentrations, especially in cells and tissues that have high levels of autofluorescence. Rhod-2 has the longest fluorescent emission signal of the commonly used calcium indicators. It contains a rhodamine-like fluorophore, whose excitation and emission maxima make it suitable for use with argon and krypton laser. Its signal intensity is the strongest of all the calcium probes. Spectral Data: λex 550nm, λem 578nm in methanol; λex 549nm, λem 578nm in DMSO; λex 550nm, λem 570nm in Chloroform:Methanol (90:10).

Product References

(1) A. Minta, et al.; J. Biol. Chem. 264, 8171 (1989) | (2) T.M. Quinton, et al.; BBRC 184, 893 (1992) | (3) M. Okada, et al.; Brain Res. 583, 227 (1992) | (4) Y. Tamura, et al.; Brain Res. 592, 317 (1992) | (5) N. Melamed, et al.; J. Neurosci. 13, 632 (1993) | (6) C. Du, et al.; Biophys. J. 80, 549 (2001) | (7) Y.N. Tallini, et al.; PNAS 103, 4753 (2006) | (8) P.R. Territo, et al.; Appl. Spectrosc. 61, 138 (2007) | (9) B.R. Choi; Methods Mol. Biol. 937, 217 (2013)

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