Rhod 2-AM

CHF 204.00
In stock
CDX-A0072-M0011 mgCHF 204.00
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Product Details
Synonyms 9-[4-[bis[2-[(Acetyloxy)methoxy]-2-oxoet; Rhod-2 AM
Product Type Chemical
Formula C52H59BrN4O19
MW 1123.96
CAS 145037-81-6
Source/Host Chemicals Synthetic.
Purity Chemicals ≥80% (HPLC)
Appearance Red-purple solid.
Solubility Soluble in DMSO, DMF, methanol or chloroform.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Smiles O=C(OCOC(C)=O)CN(CC(OCOC(C)=O)=O)C1=C(C=C(C=C1)C2=C3C=C/C(C=C3OC4=CC(N(C)C)=CC=C24)=[N+](C)/C)OCCOC5=C(C=CC(C)=C5)N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O.[Br-]
Shipping and Handling
Shipping AMBIENT
Short Term Storage -20°C
Long Term Storage -20°C
Handling Advice Keep cool and dry.
Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Long-wavelength cell-permeant Ca2+ indicator. Rhod-2/AM is an acetoxymethyl ester derivative of Rhod 2 that can be easily loaded into cells via incubation and used for quantifying intracellular Ca2+ concentration allowing non-invasive measurement of calcium ions in live cells. This fluorogenic probe for intracellular Ca2+ operates in the visible spectrum. Rhod-2/AM itself does not bind Ca2+, but it is readily hydrolyzed to Rhod-2 by endogenous esterases once the dye enters cells. Rhod-2 is a valuable alternative to Fluo-3 to investigate changes in intracellular free Ca2+ concentrations, especially in cells and tissues that have high levels of autofluorescence. Rhod-2 has the longest fluorescent emission signal of the commonly used calcium indicators. It contains a rhodamine-like fluorophore, whose excitation and emission maxima make it suitable for use with argon and krypton laser. Its signal intensity is the strongest of all the calcium probes. Spectral Data: λex 550nm, λem 578nm in methanol; λex 549nm, λem 578nm in DMSO; λex 550nm, λem 570nm in Chloroform:Methanol (90:10).

Product References

(1) A. Minta, et al.; J. Biol. Chem. 264, 8171 (1989) | (2) T.M. Quinton, et al.; BBRC 184, 893 (1992) | (3) M. Okada, et al.; Brain Res. 583, 227 (1992) | (4) Y. Tamura, et al.; Brain Res. 592, 317 (1992) | (5) N. Melamed, et al.; J. Neurosci. 13, 632 (1993) | (6) C. Du, et al.; Biophys. J. 80, 549 (2001) | (7) Y.N. Tallini, et al.; PNAS 103, 4753 (2006) | (8) P.R. Territo, et al.; Appl. Spectrosc. 61, 138 (2007) | (9) B.R. Choi; Methods Mol. Biol. 937, 217 (2013)

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