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Chemodex
Indo 1-AM
162
CHF
CHF 162.00
In stock
CDX-I0019-M0011 mgCHF 162.00
| Product Details | |
|---|---|
| Synonyms | Indo-1-(acetoxymethyl) ester; 4-(6-Carboxy-2-indolyl)-4'-methyl-2,2'-(ethylenedioxy)dianiline-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl) ester |
| Product Type | Chemical |
| Properties | |
| Formula | C47H51N3O22 |
| MW | 1009.93 |
| CAS | 112926-02-0 |
| Source/Host Chemicals | Synthetic. |
| Purity Chemicals | ≥90% (HPLC) |
| Appearance | Off-white solid. |
| Solubility | Soluble in DMSO or methanol. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | CAWBRCOBJNWRLK-UHFFFAOYSA-N |
| Smiles | CC1=CC(OCCOC2=CC(C3=CC(C=CC(C(OCOC(C)=O)=O)=C4)=C4N3)=CC=C2N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C1 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice |
Keep cool and dry. Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
INDO 1-AM is a popular non-invasive UV-excitable calcium indicator. It is the cell-permeable ester derivative of INDO 1. After crossing the cell membrane, INDO 1-AM is rapidly hydrolyzed by cytoplasmic esterases to produce the ratiometric fluorescent calcium indicator INDO 1, which remains trapped within the cell. INDO 1/AM has been used to selectively monitor Ca2+ levels in mitochondria and in the cytosol. INDO 1 is ideal for analyses using flow cytometry, as it uses a single excitation source (usually the 351-364nm spectral lines of the argon-ion laser). In contrast to FURA 1, INDO 1 has a dual emission peak. The emission of INDO 1 shifts to 400nm (when bound to Ca2+) from 480nm in Ca2+-free environments. INDO 1 is prone to photobleaching, which limits its usefulness in methods involving microscopy. Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Spectral Data: λex 330nm; λem 450nm (with Calcium), λex 356nm; λem 478nm in methanol.
Product References
(1) G. Grynkiewicz, et al.; J. Biol. Chem. 260, 3440 (1985) | (2) M. Lopez, et al.; Cytometry 10, 165 (1989) | (3) J.P. Grierson, et al.; J. Neurophysiol. 67, 704 (1992) | (4) E.J. Griffiths, et al.; Am. J. Physiol. 273, C37 (1997) | (5) R.M. Paredes, et al.; Meth. A Comp. Meth. Enzymol. 46, 143 (2008) | (6) Z. Zhou, et al.; J. Physiol. 507, 379 (1998) | (7) S. Bailey & P.J. Macardle; J. Immunol. Meth. 311, 220 (2006) | (8) A. Nelemans; Methods Mol. Biol. 312, 47 (2006) | (9) R.W. Sabnis; Handbook of biological dyes and stains (2010)
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