Indo 1-AM

CHF 162.00
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CDX-I0019-M0011 mgCHF 162.00
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Product Details
Synonyms Indo-1-(acetoxymethyl) ester; 4-(6-Carboxy-2-indolyl)-4'-methyl-2,2'-(ethylenedioxy)dianiline-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl) ester
Product Type Chemical
Formula C47H51N3O22
MW 1009.93
CAS 112926-02-0
Source/Host Chemicals Synthetic.
Purity Chemicals ≥90% (HPLC)
Appearance Off-white solid.
Solubility Soluble in DMSO or methanol.
Declaration Manufactured by Chemodex.
Other Product Data

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Shipping and Handling
Shipping AMBIENT
Short Term Storage -20°C
Long Term Storage -20°C
Handling Advice Keep cool and dry.
Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
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Product Specification Sheet
Datasheet Download PDF

INDO 1-AM is a popular non-invasive UV-excitable calcium indicator. It is the cell-permeable ester derivative of INDO 1. After crossing the cell membrane, INDO 1-AM is rapidly hydrolyzed by cytoplasmic esterases to produce the ratiometric fluorescent calcium indicator INDO 1, which remains trapped within the cell. INDO 1/AM has been used to selectively monitor Ca2+ levels in mitochondria and in the cytosol. INDO 1 is ideal for analyses using flow cytometry, as it uses a single excitation source (usually the 351-364nm spectral lines of the argon-ion laser). In contrast to FURA 1, INDO 1 has a dual emission peak. The emission of INDO 1 shifts to 400nm (when bound to Ca2+) from 480nm in Ca2+-free environments. INDO 1 is prone to photobleaching, which limits its usefulness in methods involving microscopy. Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Spectral Data: λex 330nm; λem 450nm (with Calcium), λex 356nm; λem 478nm in methanol.

Product References

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