Indo 1-AM

CHF 153.00
In stock
CDX-I0019-M0011 mgCHF 153.00
More Information
Product Details
Synonyms Indo-1-(acetoxymethyl) ester; 4-(6-Carboxy-2-indolyl)-4'-methyl-2,2'-(ethylenedioxy)dianiline-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl) ester
Product Type Chemical
Properties
Formula C47H51N3O22
MW 1009.93
CAS 112926-02-0
Source/Host Chemicals Synthetic.
Purity Chemicals ≥90% (HPLC)
Appearance Off-white solid.
Solubility Soluble in DMSO or methanol.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

InChi Key CAWBRCOBJNWRLK-UHFFFAOYSA-N
Smiles CC1=CC(OCCOC2=CC(C3=CC(C=CC(C(OCOC(C)=O)=O)=C4)=C4N3)=CC=C2N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C1
Shipping and Handling
Shipping AMBIENT
Short Term Storage -20°C
Long Term Storage -20°C
Handling Advice Keep cool and dry.
Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

INDO 1-AM is a popular non-invasive UV-excitable calcium indicator. It is the cell-permeable ester derivative of INDO 1. After crossing the cell membrane, INDO 1-AM is rapidly hydrolyzed by cytoplasmic esterases to produce the ratiometric fluorescent calcium indicator INDO 1, which remains trapped within the cell. INDO 1/AM has been used to selectively monitor Ca2+ levels in mitochondria and in the cytosol. INDO 1 is ideal for analyses using flow cytometry, as it uses a single excitation source (usually the 351-364nm spectral lines of the argon-ion laser). In contrast to FURA 1, INDO 1 has a dual emission peak. The emission of INDO 1 shifts to 400nm (when bound to Ca2+) from 480nm in Ca2+-free environments. INDO 1 is prone to photobleaching, which limits its usefulness in methods involving microscopy. Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Spectral Data: λex 330nm; λem 450nm (with Calcium), λex 356nm; λem 478nm in methanol.

Product References

(1) G. Grynkiewicz, et al.; J. Biol. Chem. 260, 3440 (1985) | (2) M. Lopez, et al.; Cytometry 10, 165 (1989) | (3) J.P. Grierson, et al.; J. Neurophysiol. 67, 704 (1992) | (4) E.J. Griffiths, et al.; Am. J. Physiol. 273, C37 (1997) | (5) R.M. Paredes, et al.; Meth. A Comp. Meth. Enzymol. 46, 143 (2008) | (6) Z. Zhou, et al.; J. Physiol. 507, 379 (1998) | (7) S. Bailey & P.J. Macardle; J. Immunol. Meth. 311, 220 (2006) | (8) A. Nelemans; Methods Mol. Biol. 312, 47 (2006) | (9) R.W. Sabnis; Handbook of biological dyes and stains (2010)

© 2017 Adipogen Life Sciences. Pictures: © 2012 Martin Oeggerli. All Rights Reserved.