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Chemodex
Calcein-AM
162
CHF
CHF 162.00
In stock
CDX-C0009-M0011 mgCHF 162.00
| Product Details | |
|---|---|
| Synonyms | CAL-AM; Calcein O,O'-diacetate tetrakis(acetoxymethyl) ester; Calcein acetoxymethyl ester; NSC 689290 |
| Product Type | Chemical |
| Properties | |
| Formula | C46H46N2O23 |
| MW | 994.86 |
| CAS | 148504-34-1 |
| Source/Host Chemicals | Synthetic. |
| Purity Chemicals | ≥95% (HPLC) |
| Appearance | White to off-white solid. |
| Solubility | Soluble in DMSO. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | XKFSBWQWNMZWFA-UHFFFAOYSA-N |
| Smiles | O=C(C1=C2C=CC=C1)OC32C4=C(C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(OC(C)=O)C=C4)OC5=C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OC(C)=O)=CC=C53 |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice |
Keep cool and dry. Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Calcein-AM is a non-fluorescent cell permeable and hydrophobic probe that, upon entering live cells, is cleaved by intracellular esterases, releasing the membrane-impermeable, hydrophilic, and intensely bright green fluorescent calcein. Better retained by viable cells than fluorescein, carboxyfluorescein, or BCECF, due to its low cytotoxicity. It can be used as an indicator of cell viability (dead cells lack cytoplasmic esterases), cell-cell communication, cytotoxicity or changes in intracellular calcium, fluoride, iron or mercury. Calcein-AM is a neutral substrate for multidrug resistance-associated protein 1 (MRP1) and multidrug resistance protein 3 (MDR3, P-glycoprotein 3) and has been used in flow cytometry studies to analyze the function of P-gp and MRP. Used as a cytosolic fluorophore in mitochondrial permeability studies to image pore transition. Viability assays using calcein are reliable and correlate well with the standard 51Cr-release assay. Spectral Data: λex 496nm; λem 516nm in 0.1M Tris pH 8.0.
Product References
(1) N. Feller, et al.; Br. J. Cancer. 72, 543 (1995) | (2) N. Feller, et al.; FEBS Lett. 368 385 (1995) | (3) F. Tiberghien & F. Loor; Anti-Cancer Drugs 7, 568 (1996) | (4) P. Decherchi, et al.; J. Neurosci. Methods 71, 205 (1997) | (5) M. Essodaigui, et al.; Biochemistry 37, 2243 (1998) | (6) J.J. Lemasters, et al.; Biochim. Biophys. Acta. 1366, 177 (1998) | (7) J. Hynes, et al.; J. Biomol. Screen. 8, 264 (2003) | (8) D. Bratosin, et al.; Cytometry A 66, 78 (2005) | (9) R.W. Sabnis; Handbook of biological dyes and stains (2010) | (10) G. Szakacs, et al.; Pathol. Oncol. Res. 4, 251 (2016)
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