IL-6 (human) (rec.) (His)

CHF 120.00
In stock
CHI-HF-20106-C01010 µgCHF 120.00
CHI-HF-20106-C05050 µgCHF 360.00
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Product Details
Synonyms Interleukin-6; B Cell Stimulatory Factor 2; BSF-2; CTL Differentiation Factor; CDF; Interferon β2; IFN-β2
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence Human IL-6 (aa 30-212) is fused at the C-terminus to a His-tag.
Crossreactivity Human
Biological Activity The ED50 as determined by the dose-dependent stimulation of the proliferation of IL-6 dependent murine 7TD1 cells is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5x 106units/mg.
MW ~22kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/μg protein (LAL test; Lonza).
Reconstitution Reconstitute 10µg vial with 100 µl sterile water to a concentration of 0.1mg/ml.
Reconstitute 50µg vial with 100 µl sterile water to a concentration of 0.5mg/ml.
Add 1X PBS to the desired protein concentration.
Formulation Lyophilized from 0.2μm-filtered solution in PBS.
Other Product Data NCBI reference NP_000591.1: IL-6 (human)
Declaration Manufactured by Chimerigen.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

Interleukin 6 (IL-6) is a pleiotropic α-helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism and cancer progression. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22kDa-28kDa phosphorylated and variably glycosylated molecule. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6R) and a signal transducing subunit (gp130). IL-6 is a key factor for the growth of plasma cells.

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