JaICA

anti-Methylglyoxal [MG], mAb (3C)

CHF 830.00

In stock

JAI-MMG-030N30 µgCHF 830.00

Figure 1: Immunohistochemical staining of MG adducts in atherosclerotic aorta using anti-MG, mAb (3C).

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Product Details
Product Type	Monoclonal Antibody
Properties
Clone	3C
Isotype	Mouse IgG2a
Immunogen/Antigen	MG-modified keyhole-lympet hemocyanine.
Application	Immunohistochemistry: Recommended concentration is 0.5-1μg/mL on paraffin sections. Western Blot (see reference Nr. 4)
Crossreactivity	All Human
Specificity	Recognizes MG-modified proteins (especially Argpyrimidine).
Purity Detail	Protein A purified.
Formulation	Frozen liquid. Contains 10mM PBS, 0.1% NaN3 and 0.5% BSA.
Isotype Negative Control	Mouse IgG2a Isotype Control
Other Product Data	Click here for Original Manufacturer Product Datasheet Our product description may differ slightly from the original manufacturers product datasheet.
Declaration	Manufactured by JaICA.
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 3 years after receipt when stored at -20°C.
Documents
MSDS	Inquire
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Methylglyoxal (MG), an endogenous metabolite that increases in diabetes and is a common intermediate in the Maillard reaction (glycation), reacts with proteins and forms advanced glycation end products (AGE). MG reacts with arginine residue in protein and forms numerous numbers of adducts, such as argpyrimidine.

Product References

Protein modification by a Maillard reaction intermediate methylglyoxal. Immunochemical detection of fluorescent 5-methylimidazolone derivatives in vivo: K. Uchida, et al.; FEBS Lett. 410, 313 (1997)
Methylglyoxal Modification of Protein -Chemical and Immunochemical Characterization of Methylglyoxal-Arginine Adduct: T. Oya, et al.; J. Biol. Chem. 274, 18492 (1999)
Advanced glycation and lipidoxidation of the peritoneal membrane: respective roles of serum and peritoneal fluid reactive carbonyl compounds: T. Miyata, et al.; Kidney Int. 58, 425 (2000)
Argpyrimidine, a blue fluorophore in human lens proteins: high levels in brunescent cataractous lenses: P.S. Padayatti, et al.; Invest. Ophthalmol. Vis. Sci. 42, 1299 (2001)
High concentrations of glucose induce synthesis of argpyrimidine in retinal endothelial cells: P.S. Padayatti, et al.; Curr. Eye Res. 23, 106 (2001)
Curcumin Inhibits ROS Formation and Apoptosis in Methylglyoxal- Treated Human Hepatoma G2 Cells: W.-H. Chan, et al.; Ann. N.Y. Acad. Sci. 1042, 372 (2005)

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