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SouthBayBio
Mpro [3CLpro] (SARS-CoV-2) (rec.) (His)
322
CHF
CHF 322.00
In stock
SBB-DE0129-C05050 µgCHF 322.00
| Product Details | |
|---|---|
| Synonyms | Chymotrypsin Like Protease; SARS Coronavirus Main Proteinase |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Sequence |
Mpro (Papain-like Protease) (aa 1540-1841) (Accession Nr. P0C6U8) is fused at the N-terminus to a His-tag. |
| Crossreactivity | Human |
| Application |
3CLpro recognizes the peptide sequence LQ[S/A/G] where it cleaves C-terminal to the amino acid glutamine. Working concentrations of this enzyme range from 10 to 100 nM using KTSAVLQ-Rh110 (SBB-PS0130) as substrate. |
| MW | ~36kDa |
| Purity | ≥98% (SDS-PAGE) |
| Concentration | Lot dependent. |
| Accession Number | P0C6U8 |
| Formulation | Liquid. In 50mM HEPES pH 7.5, 100mM NaCl, 1mM TCEP. |
| Other Product Data |
Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer |
| Declaration | Manufactured by South Bay Bio. |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -80°C |
| Long Term Storage | -80°C |
| Handling Advice | Aliquot to avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -80°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
3CLpro recognizes the peptide sequence LQ[S/A/G] where it cleaves c-terminal to the amino acid glutamine (use product SBB-PS0130, KTSAVLQ-Rh110-Glu as universal substrate). The protease 3CLpro is a potential drug target for coronavirus infections due to its essential role in processing the polyproteins that are translated from the viral RNA. The X-ray structures of the unliganded SARS-CoV-2 protease 3CLpro and its complex with an α-ketoamide inhibitor provides a basis for design of α-ketoamide inhibitors. This SARS Coronavirus recombinant 3CLpro is N-terminally His10-tagged and expressed in E.coli.
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