Chimerigen

SARS-CoV-2 Spike Protein S1 (RBD) (rec.) (GST-His)

CHF 410.00
In stock
CHI-B249001-C100100 µgCHF 410.00
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Product Details
Synonyms 2019-nCoV Spike Protein S1 (RBD)
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence

Receptor-binding domain (RBD) of SARS-CoV-2 Spike protein S1 (aa 330-530) is fused at the N-terminus to a GST-tag and at the C-terminus to a His-tag.

Crossreactivity Human
Biological Activity

Binds to the human ACE2.

MW ~50kDa (SDS-PAGE, reducing conditions)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.001EU/μg purified protein (LAL test).
Concentration 1mg/ml after reconstitution.
Reconstitution Reconstitute with 100µl endotoxin-free water.
Accession Number QHD43416.1
Formulation Lyophilized. Contains PBS.
Other Product Data

UniProt link QHD43416.1: SARS-CoV-2 S Protein

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
For maximum product recovery after thawing, centrifuge the vial before opening the cap.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
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Product Specification Sheet
Datasheet Download PDF
Description

SARS-CoV-2 shares 79.5% sequence identity with SARS-CoV and is 96.2% identical at the genome level to the bat coronavirus BatCoV RaTG133, suggesting it had originated in bats. The coronaviral genome encodes four major structural proteins: the Spike (S) protein, Nucleocapsid (N) protein, Membrane/Matrix (M) protein and the Envelope (E) protein. The SARS Envelope (E) protein contains a short palindromic transmembrane helical hairpin that seems to deform lipid bilayers, which may explain its role in viral budding and virion envelope morphogenesis. The SARS Membrane/Matrix (M) protein is one of the major structural viral proteins. It is an integral membrane protein involved in the budding of the viral particles and interacts with SARS Spike (S) protein and the Nucleocapsid (N) protein. The N protein contains two domains, both of them bind the virus RNA genome via different mechanisms. The CoV Spike (S) protein assembles as trimer and plays the most important role in viral attachment, fusion and entry. It is composed of a short intracellular tail, a transmembrane anchor and a large ectodomain that consists of a receptor binding S1 subunit (RBD domain) and a membrane-fusing S2 subunit. Sequence analysis of the SARS-CoV-2 S protein genome showed that it was only 75% identical with the SARS-CoV S protein. However, analysis of the receptor binding motif (RBM) in the S protein showed that most of the amino acid residues essential for receptor binding were conserved between SARS-CoV and SARS-CoV-2, suggesting that the 2 CoV strains use the same host receptor for cell entry, the receptor angiotensin-converting enzyme 2 (ACE2).

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