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SouthBayBio
K48-linked Penta-Ubiquitin (human) (rec.) (untagged)
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Figure 1: K48-Linked Penta-Ubiquitin SDS-PAGE. From left to right, increasing amounts of penta-ubiquitin were loaded onto a 4-20% SDS-PAGE gel, stained with Coomassie brillant blue. Purity is >95%.
| Product Details | |
|---|---|
| Synonyms | Ub5 |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Sequence |
Five enzymatically conjugated human ubiquitin (aa1-76) (Accession Nr. P0CG47) covalently linked through an isopeptide bond at K48 residue of one ubiquitin molecule and the C-terminal glycine residue of another ubiquitin molecule and untagged. |
| Crossreactivity | Human |
| MW | ~43kDa |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Purified via liquid chromatography. |
| Concentration | Lot dependent. |
| Accession Number | P0CG47 |
| Formulation | Liquid. In 50mM HEPES pH 7.5. |
| Other Product Data |
Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer Our product description may differ slightly from the original manufacturer's product datasheet. Note: Heating this product in SDS-PAGE buffer can lead to high apparent molecular weight banding or smearing on SDS-PAGE gels that is not representative of the product's purity. |
| Declaration | Manufactured by South Bay Bio. |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -80°C |
| Long Term Storage | -80°C |
| Handling Advice | Aliquot to avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -80°C. |
| Documents | |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
The array of cellular processes initiated and regulated by ubiquitin has been partially explained by the structural diversity of differently linked ubiquitin chains. In a ubiquitin chain, ubiquitin moieties can be conjugated through one of their lysine residues (K6, K11, K27, K29, K33, K48 and K63) or the N-terminal methionine residue (M1), offering countless possibilities to assemble a specific polymer. Ubiquitin molecules can also be modified by other post-translational modifications, including acetylation and phosphorylation, adding another layer of ubiquitin signal regulation and diversification. K48-polyubiquitin chains are the most abundant linkage in cells and thought to be the major signal for proteasome-mediated degradation. Quantitative MS analyses of intracellular ubiquitin linkages reveled K48-polyubiquitin linkages rapidly accumulate when cells are treated with the proteasome inhibitor MG132.
This protein is formed with wild-type human recombinant ubiquitin and linkage-specific enzymes. Ideal for investigating ubiquitin-binding proteins and as substrates for ubiquitin-specific isopeptidases. Reaction conditions will need to be optimized for each specific application. This K48-linked
penta-ubiquitin was enzymatically conjugated, and purified via liquid chromatography.
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