AdipoGen Life Sciences

anti-DLK1 (human), mAb (PF299-1) (ATTO 565)

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Product Details
Synonyms	Protein Delta Homolog 1; Fetal Antigen 1; Preadipocyte Factor 1; Pref-1; FA1
Product Type	Monoclonal Antibody
Properties
Clone	PF299-1
Isotype	Mouse IgG1κ
Immunogen/Antigen	Recombinant human DLK1 (extracellular domain).
Label/Conjugates	ATTO 565
Application	ELISA: (direct or indirect: 1:2'000-1:10'000) Flow Cytometry: (1:100-1:500) Immunohistochemistry: (paraffin sections (1:100-1:500)) Western blot: (1:2'000-1:10'000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.
Crossreactivity	Human
Specificity	Recognizes human DLK1. Does not cross-react with mouse DLK1.
Purity Detail	Protein G-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Mouse IgG1 Isotype Control
Accession Number	P80370
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

The transmembrane and secreted protein delta-like 1 homolog (DLK1; Protein delta homolog 1; Preadipocyte factor 1; Pref-1) belongs to the EGF-like family. It can be cleaved by a tumor necrosis factor-α converting enzyme (TACE) to release a soluble form. DLK1 plays important roles in regulating cell differentiation such as adipogenesis and osteogenesis. Membrane-bound DLK1 promotes hypertrophic myotube formation, whereas soluble DLK1 inhibits myotube formation. The soluble form of DLK1 inhibits preadipocyte differentiation, while membrane DLK1 represses preadipocyte proliferation. DLK1 is also associated with tumor invasion.

Product References

Increased Haematopoietic Supportive Function of USSC from Umbilical Cord Blood Compared to CB MSC and Possible Role of DLK-1: S.M. Kluth, et al.; Stem Cells Int. 2013, ID985285 (2013)
A comparative analysis of the adipogenic potential in human mesenchymal stromal cells from cord blood and other sources: M. Karagianni, et al.; Cytotherapy 15, 76 (2013)
DLK1: A Novel Target for Immunotherapeutic Remodeling of the Tumor Blood Vasculature: N.C. Sabins, et al.; Mol. Ther. 21, 1958 (2013)
A proteogenomic surfaceome study identifies DLK1 as an immunotherapeutic target in neuroblastoma: A.K. Hamilton, et al.; Cancer Cell 42, 1970 (2024)
Identification of DLK1, a Notch ligand, as an immunotherapeutic target and regulator of 1 tumor cell plasticity and chemoresistance in adrenocortical carcinoma: N.-Y. Sun, et al.; Nat. Commun. 16, 5511 (2025)

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