anti-Fas (human), mAb (APO-1-3) (preservative free)

CHF 150.00
In stock
AG-20B-0062PF-C05050 µgCHF 150.00
AG-20B-0062PF-C100100 µgCHF 240.00
AG-20B-0062PF-C500500 µgCHF 750.00
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Product Details
Synonyms CD95; APO-1; TNFRSF6; Tumor Necrosis Factor Receptor Superfamily Member 6; Apoptosis-mediating Surface Antigen FAS
Product Type Monoclonal Antibody
Clone APO-1-3
Isotype Mouse IgG3
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Recombinant human Fas.
Label/Conjugates Preservative Free

Flow Cytometry: 1-2µg/ml
Western Blot: 1:1000-1:5000
Functional Application: Induces apoptosis of SKW6.4 cells with a EC50 of 3ng/ml.
Note: Optimal conditions must be determined. For IHC use anti-Fas (human), mAb (APO-1-1) (Prod. No. AG-20B-0079PF), which is a mouse IgG1 with minimal side reactions in IHC.

Crossreactivity Human

Recognizes human Fas.

Purity ≥95% (SDS-PAGE)
Concentration 1 mg/ml.
Formulation Liquid. In PBS.
Isotype Negative Control

Mouse IgG3 Isotype Control

Other Product Data

Uniprot: Fas (human)

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Fas (CD95) is a member of the death receptor (DR) family, a subfamily of the tumor necrosis factor receptor superfamily. The formation of the Fas death-inducing signaling complex (DISC) is the initial step of Fas signaling. Activation of procaspase-8 at the DISC leads to the induction of DR-mediated apoptosis. Stimulation of Fas has been also reported to trigger non-apoptotic pathways. It has been shown that membrane-bound FasL is essential for the cytotoxic activity, whereas soluble FasL appears to promote autoimmunity and tumorigenesis via induction of non-apoptotic pathways, in particular NF-κB.

Product References
  1. Monoclonal antibody-mediated tumor regression by induction of apoptosis: B.C. Trauth, et al.; Science 245, 301 (1989)
  2. Monoclonal-antibody-mediated apoptosis in adult T-cell leukaemia: K.M. Debatin, et al.; Lancet 335, 497 (1990)
  3. Induction of apoptosis by monoclonal antibody anti-APO-1 class switch variants is dependent on cross-linking of APO-1 cell surface antigens: J. Dhein, et al.; J. Immunol. 149, 3166 (1992)
  4. APO-1-induced apoptosis of leukemia cells from patients with adult T-cell leukemia: K.M. Debatin, et al.; Blood 81, 2972 (1993)
  5. The apoptosis-1/Fas protein in human systemic lupus erythematosus: E. Mysler, et al.; J. Clin. Invest. 93, 1029 (1994)
  6. Inhibition of apoptosis in T cells expressing human T cell leukemia virus type I Tax: K.F. Copeland, et al.; AIDS Res. Hum. Retroviruses 10, 1259 (1994)
  7. Activation induces sensitivity toward APO-1 (CD95)-mediated apoptosis in human B cells: P.T. Daniel & P.H. Krammer; J. Immunol. 152, 5624 (1994)
  8. APO-1 (CD95) mediated apoptosis in human T-ALL engrafted in SCID mice: K.M. Lücking-Famira, et al.; Leukemia 8, 1825 (1994)
  9. Apoptotic cell death induced by a mouse-human anti-APO-1 chimeric antibody leads to tumor regression: L.R. Coney, et al.; Int. J. Cancer 58, 562 (1994)
  10. Cell nucleus and DNA fragmentation are not required for apoptosis: K. Schulze-Osthoff, et al.; J. Cell Biol. 127, 15 (1994)
  11. Divergent signalling via APO-1/Fas and the TNF receptor, two homologous molecules involved in physiological cell death: K. Schulze-Osthoff, et al.; EMBO J. 13, 4587 (1994)
  12. Autocrine T-cell suicide mediated by APO-1/(Fas/CD95): J. Dhein, et al.; Nature 373, 438 (1995)
  13. CD40 ligation induces Apo-1/Fas expression on human B lymphocytes and facilitates apoptosis through the Apo-1/Fas pathway: E.J. Schattner, et al.; J. Exp. Med. 182, 1557 (1995)
  14. Cytotoxicity-dependent APO-1 (Fas/CD95)-associated proteins form a death-inducing signaling complex (DISC) with the receptor: F.C. Kischkel, et al.; EMBO J. 14, 5579 (1995)
  15. Resistance to APO-1 (CD95) induced apoptosis in T-ALL is determined by a BCL-2 independent anti-apoptotic program: K.M. Debatin & P.H. Krammer; Leukemia 9, 815 (1995)
  16. Sensitization of T cells to CD95-mediated apoptosis by HIV-1 Tat and gp120: M.O. Westendorp, et al.; Nature 375, 497 (1995)
  17. The medical significance of physiological cell death: G. Hacker & D.L. Vaux; Med. Res. Rev. 15, 299 (1995)
  18. Involvement of the CD95 (APO-1/FAS) receptor/ligand system in drug-induced apoptosis in leukemia cells: C. Friesen, et al.; Nat. Med. 2, 574 (1996)
  19. Fas-threshold signalling in MSCs promotes pancreatic cancer progression and metastasis: A. Mohr, et al.; Cancer Lett. 519, 63 (2021)
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