Cookie Policy: This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.
AdipoGen Life Sciences
anti-β-Tubulin, mAb (AXO45)
400
CHF
CHF 400.00
In stock
AG-20B-0085-C100100 µgCHF 400.00
Figure 1: Immunoblot analysis of β-tubulin protein glutamylation using anti-β-Tubulin, mAb (AXO45) (Prod. No. AG-20B-0085). Method: HEK-293T cells are grown in standard culture conditions, transfected with plasmids expressing the different glutamylases, TTLL4 and TTLL6 and are run on a 10% SDS-PAGE (specific protocol to separate α- and β-tubulin (Magiera & Janke, 2013)). The proteins are transferred to a nitrocellulose membrane and detected by standard immunoblot protocol using the AXO45 (1:1'000) in PBS containing 0.1% Tween-20 for washing steps and 2.5% fat free milk for antibody incubation. In all the cell extracts, AXO45 specifically detects β-tubulin, with no cross-reactivity to α-tubulin. Glutamylation added by either TTLL4 or TTLL6 appears to not affect this detection. Picture courtesy of Sudarshan Gadadhar, Shreyangi Chakraborty, Mariya Genova and Carsten Janke, Institut Curie, Orsay.
| Product Details | |
|---|---|
| Synonyms | Tubulin β Chain; Tubulin β-5 Chain |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | AXO45 |
| Isotype | Mouse IgG1 |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Axonemal tubulin from the ciliate Paramecium electroeluted from the 55 kDa band of the SDS-PAGE gel. |
| Application |
Immunocytochemistry (1:500) Immunohistochemistry Immunoprecipitation Western Blot (1:1000) Optimal conditions must be determined individually for each application. |
| Crossreactivity |
Ciliates Human Mouse |
| Specificity |
Detects almost all isotypes of β-tubulin specifically in cells and tissues of mouse, human and ciliates. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein A-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 0.02% Proclin 300. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability |
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The β-tubulin monoclonal antibody (AXO45) was developed by isolating the cilia from unicellular multiciliated Paramecium and purifying the core microtubule-based structures of the cilia, the axonemes. These axonemes were electrophoresed on a SDS-PAGE and the tubulin band was used as an antigen to develop monoclonal antibodies targeting both α- and β-tubulin (A.M. Callen, et al.; 1994). The antibody AXO45 recognizes the C-terminal region between residues 257 and 442 of β-tubulin. Although the antibody was raised against the β-tubulin found in cilia, it is able to recognize β-tubulin of all microtubule networks, indicating that it can potentially recognize different isotypes of β-tubulin found in mammals.
Product References
- Isolation and characterization of libraries of monoclonal antibodies directed against various forms of tubulin in Paramecium: A.M. Callen, et al.; Biol. Cell 81, 95 (1994)
- A targeted multienzyme mechanism for selective microtubule polyglutamylation: J. van Dijk, et al.; Mol. Cell 26, 437 (2007)
- Investigating tubulin posttranslational modifications with specific antibodies: M.M. Magiera, et al.; Methods Cell Biol. 115, 247 (2013)
Related Products
