AdipoGen Life Sciences

anti-β-Tubulin, mAb (AXO45)

CHF 400.00
In stock
AG-20B-0085-C100100 µgCHF 400.00
More Information
Product Details
Synonyms Tubulin β Chain; Tubulin β-5 Chain
Product Type Monoclonal Antibody
Properties
Clone AXO45
Isotype Mouse IgG1
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Axonemal tubulin from the ciliate Paramecium electroeluted from the 55 kDa band of the SDS-PAGE gel.
Application

Immunocytochemistry (1:500)

Immunohistochemistry

Immunoprecipitation

Western Blot (1:1000)

Optimal conditions must be determined individually for each application.

Crossreactivity Ciliates
Human
Mouse
Specificity

Detects almost all isotypes of β-tubulin specifically in cells and tissues of mouse, human and ciliates.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein A-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS containing 0.02% Proclin 300.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 month after receipt when stored at +4°C.
Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

The β-tubulin monoclonal antibody (AXO45) was developed by isolating the cilia from unicellular multiciliated Paramecium and purifying the core microtubule-based structures of the cilia, the axonemes. These axonemes were electrophoresed on a SDS-PAGE and the tubulin band was used as an antigen to develop monoclonal antibodies targeting both α- and β-tubulin (A.M. Callen, et al.; 1994). The antibody AXO45 recognizes the C-terminal region between residues 257 and 442 of β-tubulin. Although the antibody was raised against the β-tubulin found in cilia, it is able to recognize β-tubulin of all microtubule networks, indicating that it can potentially recognize different isotypes of β-tubulin found in mammals. 

Product References
  1. Isolation and characterization of libraries of monoclonal antibodies directed against various forms of tubulin in Paramecium: A.M. Callen, et al.; Biol. Cell 81, 95 (1994)
  2. A targeted multienzyme mechanism for selective microtubule polyglutamylation: J. van Dijk, et al.; Mol. Cell 26, 437 (2007)
  3. Investigating tubulin posttranslational modifications with specific antibodies: M.M. Magiera, et al.; Methods Cell Biol. 115, 247 (2013)
© 2017 Adipogen Life Sciences. Pictures: © 2012 Martin Oeggerli. All Rights Reserved.