AdipoGen Life Sciences

anti-Nampt (Visfatin/PBEF) (human), pAb (CT)

CHF 450.00
In stock
AG-25A-0027-C100100 µgCHF 450.00
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Product Details
Synonyms Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase
Product Type Polyclonal Antibody
Properties
Source/Host Rabbit
Immunogen/Antigen Synthetic peptide corresponding to aa 413-431 (V413FKDPVADPNKRSKKGRLS431) of human Nampt (Visfatin/PBEF).
Application

ELISA: (direct and indirect: 1:2’000-1:5’000).
Immunohistochemistry: (paraffin sections, 10µg/ml).
Western Blot: (1:2’000-1:5’000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.

Crossreactivity Human
Specificity

Recognizes human Nampt. Weakly cross-reacts with mouse Nampt. Detects a band of ~54kDa by Western blot.

Purity Detail Protein A-affinity purified.
Concentration 1mg/ml
Formulation Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control

Rabbit IgG

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

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