AdipoGen Life Sciences

anti-Nampt (Visfatin/PBEF) (human), pAb (CT)

CHF 450.00

In stock

AG-25A-0027-C100100 µgCHF 450.00

Figure 1: Western blot analysis using anti-Nampt (human), pAb (Prod. No. AG-25A-0027) at 1:2'000 dilution.
1: Human Nampt (His-tagged).
2: LPS-treated human peripheral blood leukocyte lysate.

Figure 2: Immunohistochemical staining of Nampt using anti-Nampt (Visfatin/PBEF) (human), pAb (CT) (Prod. No. AG-25A-0027) in formalin-fixed and paraffin-embedded (FFPE) human colon tissue (10µg/ml).

Specifications / Handling

More Information
Product Details
Synonyms	Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase
Product Type	Polyclonal Antibody
Properties
Source/Host	Rabbit
Immunogen/Antigen	Synthetic peptide corresponding to aa 413-431 (V⁴¹³FKDPVADPNKRSKKGRLS⁴³¹) of human Nampt (Visfatin/PBEF).
Application	ELISA: (direct and indirect: 1:2’000-1:5’000). Immunohistochemistry: (paraffin sections, 10µg/ml). Western Blot: (1:2’000-1:5’000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Crossreactivity	Human
Specificity	Recognizes human Nampt. Weakly cross-reacts with mouse Nampt. Detects a band of ~54kDa by Western blot.
Purity Detail	Protein A-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Rabbit IgG
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

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