AdipoGen Life Sciences

anti-NLRC5 (mouse), pAb (IN113)

CHF 250.00
In stock
AG-25B-0038-C100100 µgCHF 250.00
More Information
Product Details
Synonyms NOD-like Receptor Family CARD Domain Containing 5; Caterpiller Protein 16.1; NOD4; Nucleotide-binding Oligomerization Domain Protein 4
Product Type Polyclonal Antibody
Properties
Source/Host Rabbit
Immunogen/Antigen Recombinant mouse NLRC5 (CARD domain; aa1-139).
Application

Western Blot: (1:500)*

CHIP: (see Reference 1)

*Note: We recommend a starting dilution of 1:500 and to optimize the dilution depending on your cells/tissues.

Crossreactivity Mouse
Specificity

Recognizes full-length mouse NLRC5. Does not cross-react with human NLRC5.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein A affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Isotype Negative Control

Rabbit IgG

Other Product Data

UniProt link C3VPR6: NLRC5 (mouse)

Accession Number C3VPR6
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Major histocompatibility complex class I molecules are expressed in all nucleated cells, whereas MHC class II molecules are mostly expressed in hematopoietic cells. The tight regulation of both MHC class I and class II molecules is crucial for eliciting an appropriate immune response. The MHC class II and class I molecule regulation is controlled by class II MHC transactivator (CIITA) and by NLR family caspase recruitment domain-containing 5 (NLRC5), respectively.

NOD-like receptor family CARD domain containing 5 (NLRC5) is the largest member of the NOD-like receptors (NLRs) family. As all NLR-family members, NLRC5 shares a similar protein structure consisting of three main domains: a variable N-terminal protein-interaction domain, a central Nucleotide-Binding Domain (NBD) and a C-terminal stretch consisting of Leucine Rich Repeats (LRRs). NLRC5 contains between its CARD and NBD domain a bipartite-type nuclear localization signal (NLS), allowing NLRC5 transport towards the nucleus. The expression levels of NLRC5 and MHC class I are highly correlated, with more NLRC5 resulting in more MHC class I expression and increased MHC class I cell surface levels. NLRC5 is highly expressed in hematopoietic cells and tissues such as the spleen, lymph node, thymus, intestine, lung and bone marrow. NLRC5 is inducible by interferon-γ (IFN-γ). NLRC5 is decreased in tumor tissues of most cancer types. Lower NLRC5 expression is associated with reduced survival in melanoma, rectal, bladder, uterine, cervical and head/neck cancer.

Product References
  1. NLRC5 Deficiency Selectively Impairs MHC Class I- Dependent Lymphocyte Killing by Cytotoxic T Cells: F. Staehli, et al.; J. Immunol. 188, 3820 (2012)
  2. NLRC5 Exclusively Transactivates MHC Class I and Related Genes through a Distinctive SXY Module: K. Ludigs, et al.; PLoS Genet. 11, e1005088 (2015)
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