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AdipoGen Life Sciences
Irisin (rec.) (CHO)
380
CHF
CHF 380.00
In stock
AG-40B-0136-C01010 µgCHF 380.00
AG-40B-0136-30103 x 10 µgCHF 760.00
| Product Details | |
|---|---|
| Synonyms | Fibronectin Type III Domain-containing Protein 5 (cleaved); Fibronectin Type III Repeat-containing Protein 2 (cleaved); FNDC5 (cleaved) |
| Product Type | Protein |
| Properties | |
| Source/Host | CHO cells |
| Sequence |
Irisin (human, aa 32-143 /mouse, aa 29-140) is FLAG®-tagged at the N-terminus. Irisin has 100% identity between mouse, rat, monkey and human. Monomeric. Purified by Size Exclusion Chromatography (SEC). |
| Crossreactivity |
Human Mouse |
| Biological Activity |
Activates brown adipocyte markers Ucp1 and Cidec in murine subcutaneous adipocytes at 10ng/ml [Lit. 1]. |
| MW | ~26kDa |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg purified protein (LAL test). |
| Concentration | 0.1mg/ml after reconstitution. |
| Reconstitution | Reconstitute with 100μl sterile water. |
| Formulation | Lyophilized. Contains PBS. |
| Other Product Data |
Uni-Prot link Q8NAU1: Irisin |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Irisin is a recently described exercise-induced hormone secreted by skeletal muscle in mice and humans. Irisin activates beige fat cells (beige cells have a gene expression pattern distinct from either white or brown fat and are preferentially sensitive to the polypeptide hormone Irisin). Irisin is cleaved from the type I membrane protein FNDC5 and improves systemic metabolism by increasing energy expenditure. Neuroprotectant in Alzheimer's disease.
This protein is purified by size exclusion chromatography (SEC) and contains only the monomeric form and no aggregates.
Product References
- Leptin administration activates irisin-induced myogenesis via nitric oxide-dependent mechanisms, but reduces its effect on subcutaneous fat browning in mice: A. Rodriguez, et al.; Int. J. Obes. 39, 397 (2015)
- Exercise-linked FNDC5/irisin rescues synaptic plasticity and memory defects in Alzheimer’s models: M.V. Lourenko, et al.; Nat. Med. 25, 165 (2019)
- Irisin levels in genetic and essential obesity: clues for a potential dual role: S. Mai, et al.; Nature Sci. Rep. 10, 1020 (2020)
- Exercise hormone irisin is a critical regulator of cognitive function: M.R. Islam, et al.; Nat. Metab. 3, 1058 (2021)
- Renal protection induced by physical exercise may be mediated by the irisin/AMPK axis in diabetic nephropathy: G.P. Formigiari, et al.; Nature Sci. Rep. 12, 9062 (2022)
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