Nampt (mouse) (rec.) (enzymatically active)

CHF 300.00
In stock
AG-40B-0179-C01010 µgCHF 300.00
AG-40B-0179-30103 x 10 µgCHF 600.00
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Product Details
Synonyms Visfatin; PBEF; Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase; NAmPRTase
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence

Mouse Nampt (visfatin/PBEF) (aa 1-491) is fused at the N-terminus to a FLAG®-tag.

Crossreactivity Mouse
Application

Enzymatically active to convert nicotinamide + phosphoribosyltransferase (PRPP) to nicotinamide mononucleotide (NMN).

Biological Activity

0.5µg to 5µg of Nampt (mouse) (rec.) (enzymatically active) show activity in a colorimetric Nampt assay.

MW ~55kDa (SDS-PAGE)
Purity ≥90% (SDS-PAGE)
Endotoxin Content <0.01EU/μg purified protein (LAL test; Lonza).
Concentration Lot dependent.
Formulation Liquid. Contains PBS.
Other Product Data

UniProt link Q99KQ4: Nicotinamide phosphoribosyltransferase (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.

Shipping and Handling
Shipping DRY ICE
Short Term Storage -20°C
Long Term Storage -80°C
Handling Advice After opening, prepare aliquots and store at -80°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 6 months after receipt when stored at -80°C.
Documents
MSDS No
Product Specification Sheet
Datasheet Download PDF

Nicotinamide phosphoribosyltransferase (NAmPRTase or Nampt) also known as pre-B cell colony-enhancing factor 1 (PBEF1) or visfatin is the rate-limiting enzyme in the nicotinamide adenine dinucleotide (NAD+) salvage pathway that converts nicotinamide to nicotinamide mononucleotide in mammals to enable NAD+ biosynthesis. In addition it has been shown to be an adipokine that is localized in the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. The recombinant Nampt (mouse) (rec.) (enzymatically active) (Prod. No. AG-40B-0179) shows activity to convert nicotinamide + phosphoribosyltransferase (PRPP) to nicotinamide mononucleotide (NMN). It migrates as a homodimer on a size exclusion chromatography (SEC) column. 

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