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AdipoGen Life Sciences
Fc (LALA-PG)-KIH (human):IL-1Ra (mouse) (monomeric) (rec.)
440
CHF
CHF 440.00
In stock
AG-40B-0254-C05050 µgCHF 440.00
Figure 1: Fc (LALA-PG)-KIH (human):IL-1Ra (mouse) (monomeric) (rec.) (#AG-40B-0254) binds to the receptor mouse IL-1R1. Method: Mouse IL-1R1 is coated on an ELISA plate at 1 µg/ml overnight at room temperature. Fc (LALA-PG)-KIH (human):IL-1Ra (mouse) (monomeric) (rec.), or the control protein Fc (LALA-PG)-KIH (human) IgG1 Control (rec.) (#AG-35B-0018) are added (starting at 8000 ng/ml with a two-fold serial dilution) during one hour and then detected using an anti-human Fc (HRP) antibody.
Figure 2: Fc (LALA-PG)-KIH (human):IL-1Ra (mouse) (monomeric) (rec.) (#AG-40B-0254) inhibits mouse IL-1β binding to its receptor mouse IL-1R1. Method: Mouse IL-1β is coated on an ELISA plate at 2µg/ml overnight at room temperature. Fc (LALA-PG)-KIH (human):IL-1Ra (mouse) (monomeric) (rec.) (#AG-40B-0254) or the control protein Fc (LALA-PG)-KIH (human) IgG1 Control (rec.) (#AG-35B-0018) is added (starting at 8000ng/ml with a twofold serial dilution) together with IL-1R1 (mouse) (His) (1µg/ml) during one hour and the binding is then detected using an anti-His-Biotin antibody followed by addition of Streptavidin-HRP. The binding is finally observed by addition of TMB (3,3',5,5' tetramethylbenzidine).
Figure 3: Protein structure of Fc (LALA-PG)-KIH (human):IL-1Ra (mouse) (monomeric) (rec.) (Prod. No. AG-40B-0254).
| Product Details | |
|---|---|
| Synonyms | Fc (LALA-PG)-KIH:Interleukin-1 Receptor Antagonist (mouse); IL-1Ra; Interleukin-1R Antagonist |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells. Produced using animal component-free medium. |
| Sequence |
The extracellular domain of mouse IL-1Ra (aa 27-178) is fused to the C-terminus of the Fc (LALA-PG) Knob region of human IgG1. Fc (LALA-PG) Knobs:IL-1Ra (mouse) and Fc (LALA-PG) Holes form the Fc (LALA-PG)-KIH (human):IL-1Ra (mouse) (rec.) using the Knobs-into-Holes technology (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)). |
| Crossreactivity | Mouse |
| Specificity |
Binds to mouse IL-1R1. Fc (LALA-PG)-KIH (human) does not bind to FcRs. |
| Biological Activity |
It inhibits the binding of IL-1α and IL-1β to the receptor IL-1R. The Fc contains the mutations LALA-PG that abolish the interaction between the Fc and FcγRs and therefore Fc undesirable effects. |
| MW | ~50kDa and 30 kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.005 EU/μg purified protein (LAL test). |
| Concentration | 1mg/ml after reconstitution. |
| Reconstitution | Reconstitute with 50μl endotoxin-free water. |
| Accession Number | PP25085 |
| Formulation | Lyophilized. Contains PBS |
| Protein Negative Control |
Fc (LALA-PG)-KIH (human) IgG1 Control (rec.) (Prod. No. AG-35B-0018) |
| Other Product Data |
NCBI reference P25085: IL-1Ra (mouse) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
IL-1 is a group of inflammatory cytokines, represented by interleukin-1α and interleukin-1β. IL-1Ra is a cytokine initially isolated from macrophages. It shares 19% of homologous amino acids with IL1A and 30% of homologous amino acids with IL1B. IL-1Ra is abundant in the proximal digestive tract reproductive system, skin, bone marrow, and liver. IL-1Ra is the principal endogenous IL-1 antagonist. IL-1Ra is upregulated during host acute or chronic inflammatory responses. IL-1Ra plays diverse roles in anti-inflammatory and anti-tumor processes, either inhibiting tumor angiogenesis and proliferation or suppressing tumor migration and metastasis.
The protein Fc (LALA-PG)-KIH (human):IL-1Ra (mouse) (monomeric) (rec.) is produced by using two different vectors, one encoding for the Fc Knobs (LALA-PG) (human):IL-1Ra (mouse) sequence (synthesizing a protein of 50kDa) and one encoding for the Fc Holes (LALA-PG) sequence (synthesizing a protein of 30kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization and for secretion of the final protein Fc (LALA-PG)-KIH (human):IL-1Ra (mouse) (monomeric) (rec.). This Fc-KIH format allows our mouse IL-1Ra protein to form a monomer that is the most active structure to bind and inhibit the IL-1 receptor. The LALA-PG mutations inhibit binding to FcγRs and C1q while FcRn binding and Fc stability remain unaffected.
InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research, developed and manufactured by AdipoGen Life Sciences.
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