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AdipoGen Life Sciences
IL-15 (mouse):IL-15Ra (Sushi) (mouse):Fc (LALA-PG)-KIH (human) (rec.)
440
CHF
CHF 440.00
In stock
AG-40B-0257-C05050 µgCHF 440.00
Figure 1: Protein structure of IL-15 (mouse):IL-15Ra (Sushi) (mouse):Fc (LALA-PG)-KIH (human) (rec.) (Prod. No. AG-40B-0257).
Figure 2: IL-15 (mouse):IL-15Ra (Sushi) (mouse):Fc (LALA-PG)-KIH (human) (rec.) (#AG-40B-0257) increases proliferation of CTLL-2 mouse cytotoxic T cells.
Method: Activity of IL-15 (mouse):IL-15Ra (Sushi) (mouse):Fc (LALA-PG)-KIH (human) (rec.) is measured using proliferation of CTLL-2 cells. CTLL-2 are grown in RPMI medium, glutamine, 10% FBS, 100 IU IL-2 (#AG-40B-0224) and different concentrations of IL-15 (mouse):IL-15Ra (Sushi) (mouse):Fc (LALA-PG)-KIH (human) (rec.) (#AG-40B-0257) or the control protein Fc (LALA-PG)-KIH (human) IgG1 Control (rec.) (#AG-35B-0018). Cell proliferation is quantified using PMS/MTS (CellTiter 96® AQueous One Solution Cell Proliferation Assay, Promega).
Method: Activity of IL-15 (mouse):IL-15Ra (Sushi) (mouse):Fc (LALA-PG)-KIH (human) (rec.) is measured using proliferation of CTLL-2 cells. CTLL-2 are grown in RPMI medium, glutamine, 10% FBS, 100 IU IL-2 (#AG-40B-0224) and different concentrations of IL-15 (mouse):IL-15Ra (Sushi) (mouse):Fc (LALA-PG)-KIH (human) (rec.) (#AG-40B-0257) or the control protein Fc (LALA-PG)-KIH (human) IgG1 Control (rec.) (#AG-35B-0018). Cell proliferation is quantified using PMS/MTS (CellTiter 96® AQueous One Solution Cell Proliferation Assay, Promega).
| Product Details | |
|---|---|
| Synonyms | InVivoKine™; IL-15 (mouse):IL-15Ra (Sushi) (mouse):Fc (LALA-PG) Knobs-into-Holes (human) (rec.); Interleukin-15:Interleukin-15Ra (Sushi Domain) |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells. Produced using animal component-free medium. |
| Sequence |
IL-15 (aa 49-162) (mouse):Fc (LALA-PG) (human) Knobs and IL-15Ra (Sushi Domain) (aa 34-98) (mouse):Fc (LALA-PG) (human) Holes form the IL-15 (mouse):IL-15Ra (Sushi) (mouse):Fc (LALA-PG)-KIH (human) using the Knobs-into-Holes technology (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)). |
| Crossreactivity | Mouse |
| Biological Activity |
Induces mouse CTLL2 (T cell line) proliferation with an ED50 of <300pM. |
| MW | ~50kDa and 40kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.001EU/μg purified protein (LAL test) |
| Concentration | 1mg/ml after reconstitution. |
| Reconstitution | Reconstitute with 50μl sterile water. |
| Accession Number | P48346, Q60819 |
| Formulation | Lyophilized. Contains PBS |
| Protein Negative Control |
Fc (LALA-PG)-KIH (human) IgG1 Control (rec.) (Prod. No. AG-35B-0018) |
| Other Product Data |
UniProt link P48346: IL-15 (mouse) UniProt link Q60819: IL-15Ra (mouse) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Interleukin-15 (IL-15) is a cytokine that belongs to the interleukin-2 (IL-2) family and is essential for the development, proliferation and activation of immune cells, including natural killer (NK) cells, T cells and B cells. IL-15, unlike other cytokines, is widely expressed by many cell types including monocytes, macrophages, DC, fibroblasts, epithelial cells and skeletal muscle cells, but surprisingly not by T cells. IL-15 signals through a heterotrimeric receptor that is composed of the common γ chain (γc) subunit (CD132), the β chain (βc) subunit (IL-2/IL-15R, CD122) and the IL-15 specific α subunit IL-15Rα (CD215). IL-15 binding to its trimeric receptor induces JAK1 activation which phosphorylates STAT3 via the beta chain, and JAK3 activation which phosphorylates STAT5 via the γ chain. IL-15, like IL-2, stimulates the proliferation of T cells, induces the generation of cytotoxic lymphocytes and memory phenotype CD8 T cells, and stimulates the proliferation and maintenance of natural killer (NK) cells. In contrast to IL-2, IL-15 does not mediate activation-induced cell death (AICD) and does not consistently activate Tregs. If IL-2 is a promiscuously secreted molecule, IL-15 is locally secreted in small quantities where membrane-bound IL-15 induces signals at an immunological synapse. IL-15Rα (especially its sushi domain) acts more like a stabilizing and synergistic element (chaperone molecule) of IL-15, assisting IL-15 in its trans-presentation. The new recombinant protein IL-15 (mouse):IL-15Ra (Sushi) (mouse):Fc (LALA-PG)-KIH (human) (rec.) is an enhanced form of IL-15 with an extended half-life that stimulates the proliferation of T cells, B cells and NK cells in vivo, and increases its anti-tumor activity.
The protein IL-15 (mouse):IL-15Ra (Sushi) (mouse):Fc (LALA-PG)-KIH (human) (rec.) is produced by using two different vectors, one encoding for the IL-15 (mouse):Fc (LALA-PG) Knobs sequence (synthesizing a protein of 50kDa) and one encoding for the IL-15Ra (Sushi) (mouse):Fc (LALA-PG) Holes sequence (synthesizing a protein of 40kDa). Both vectors transfected into HEK 293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization and for secretion of the final protein IL-15 (mouse):IL-15Ra (Sushi) (mouse):Fc (LALA-PG)-KIH (human) (rec.). The LALA-PG mutations inhibit binding to FcγRs and C1q while FcRn binding and Fc stability remain unaffected.
InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research, developed and manufactured by AdipoGen Life Sciences.
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