AdipoGen Life Sciences

IL-10 (mouse):Fc (mouse) (rec.) (non-lytic)

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AG-40B-0286-C05050 µgCHF 440.00

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Product Details
Synonyms	Interleukin-10; Cytokine Synthesis Inhibitory Factor; CSIF
Product Type	Protein
Properties
Source/Host	CHO cells
Sequence	The extracellular domain of mouse IL-10 (aa 19-178) is fused to the N-terminus of the Fc region of a mutant mouse IgG2a.
Crossreactivity	Mouse
Specificity	Binds to mouse IL-10 receptor (IL-10R) complex.
Biological Activity	Inhibits mouse IL-6 secretion induced by LPS in RAW 264.7 cells.
Purity	≥98% (SDS-PAGE)
Endotoxin Content	<0.02 EU/μg protein (LAL test).
Concentration	After reconstitution: 1mg/ml
Reconstitution	Reconstitute with 50μl endotoxin-free water.
Accession Number	P18893
Formulation	Lyophilized. Contains PBS.
Protein Negative Control	Fc (mouse) IgG2a Control (rec.) (non-lytic)
Other Product Data	Non-lytic: Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC). Uniprot link P18893: IL-10 (mouse)
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Interleukin-10 (IL-10) is a homodimeric cytokine. Its functional form consists of two identical monomers, each composed of approximately 160 amino acids. These monomers are arranged in a noncovalent dimer, stabilized by hydrophobic and electrostatic interactions. This dimeric structure is essential for IL-10's ability to bind its receptor complex, which includes IL-10 receptor α (IL-10R1) and IL-10 receptor β (IL-10R2), to mediate its anti-inflammatory and immunoregulatory functions.

IL-10 is a cytokine with multiple, pleiotropic effects in immunoregulation and inflammation. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B-cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity and is involved in the regulation of the JAK-STAT signaling pathway.

IL-10 is an immunosuppressive cytokine as it plays an important regulatory role in limiting excessive immune response to inflammation and in protection against autoimmune diseases. Through the downregulation of major histocompatibility complex (MHC-II), IL-10 blunts dendritic cell (DC) response to antigen stimulation, thus inhibiting the production of several stimulatory cytokines. IL-10 can enhance the survival of B cells and cytotoxic T cells, aiding in the immune response to certain viral infections. IL-10 also inhibits CD4+ T cells’ proliferation and function, thus contributing to an immunosuppressive tumor microenvironment.

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