AdipoGen Life Sciences

FNDC4 (human) ELISA Kit

CHF 610.00
In stock
AG-45B-0028-KI0196 wellsCHF 610.00
More Information
Product Details
Synonyms Fibronectin Type III Domain-containing Protein 4; Fibronectin Type III Repeat-containing Protein 1; Fc Soluble FNDC4; FcsFNDC4
Product Type Kit
Properties
Application Set Quantitative ELISA
Specificity

Detects human FNDC4 in serum, plasma and cell culture supernatant. It cross-reacts with mouse, rat, monkey and dog FNDC4. It does not cross-react with human, mouse or rat FNDC5 / Irisin.

Crossreactivity Dog
Human
Monkey
Mouse
Rat
Quantity

1 x 96 wells

Sensitivity 40pg/ml
Range 0.078 to 5ng/ml
Sample Type Cell Culture Supernatant
Plasma
Serum
Assay Type Sandwich
Detection Type Colorimetric
Other Product Data

UniProt link Q9H6D8: FDNC4 (human)

Accession Number Q9H6D8
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice After standard reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Plate and reagents should reach room temperature before use.
Use/Stability 12 months after the day of manufacturing. See expiry date on ELISA Kit box.
Documents
Manual Download PDF
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Irisin is a recently described exercise-induced hormone secreted by skeletal muscle in mice and humans. Irisin activates beige fat cells (beige cells have a gene expression pattern distinct from either white or brown fat and are preferentially sensitive to the polypeptide hormone irisin). Irisin is cleaved from the type I membrane protein FNDC5 and improves systemic metabolism by increasing energy expenditure. FNDC4 is an ortholog of FNDC5 with 50% identity and 86% similarity compared to Irisin. FNDC4 as well as FNDC5 are extremely well conserved between species. The human FNDC4 gene is highly enriched in liver, brain tissue and adipocytes. FNDC4 is a factor with direct therapeutic potential in inflammatory bowel disease and possibly other inflammatory diseases. 

Recently, a new role of FNDC4 as a hepatokine has been published. Liver primarily controls the circulating levels of FNDC4 showing tight correlation with insulin sensitivity. In addition, a new orphan adhesion G protein-coupled receptor 116 (GPR116) has been identified as a receptor of FNDC4 in white adipose tissue (WAT), thereby establishing an endocrine FNDC4-GPR116 axis in the control of systemic glucose homeostasis. Moreover, the FNDC4-GPR116 axis is impaired in diabetic patients and therapeutic injections of recombinant Fc-FNDC4 into pre-diabetic mice corrected pre-diabetic hyperglycemia.

FNDC4 is a factor with direct therapeutic potential in inflammatory bowel disease and possibly other inflammatory diseases and is a potential biomarker for different inflammatory, metabolic diseases as well as some cancers (e.g. Glioblastoma). 

© 2017 Adipogen Life Sciences. Pictures: © 2012 Martin Oeggerli. All Rights Reserved.