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AdipoGen Life Sciences
Ac-Arg-Leu-Arg-AMC
75
CHF
CHF 75.00
In stock
AG-CP3-0013-M0011 mgCHF 75.00
AG-CP3-0013-M0055 mgCHF 300.00
| Product Details | |
|---|---|
| Synonyms | Ac-RLR-AMC . TFA; Proteasome Substrate |
| Product Type | Chemical |
| Properties | |
| Formula |
C30H46N10O6 . C2HF3O2 |
| MW | 642.8 . 114.0 |
| Sequence |
Acetyl-Arg-Leu-Arg-7-amido-4-methylcoumarin |
| CAS | 929903-87-7 (free base) |
| Purity Chemicals | ≥97% (HPLC) |
| Appearance | Solid lyophilized powder. |
| Solubility | Soluble in 5% acetic acid. |
| Identity | Determined by MS. |
| Other Product Data |
Use: Add from DMSO stock directly to in vitro or in vivo assay at desired concentration. Typical concentrations range from 10-100 μM. Activity with purified proteasome: Km=78μM. |
| InChi Key | CEEMPRHZPRGAAK-RGRVRPFLSA-N |
| Smiles | CC(C)C[C@H](NC(=O)[C@H](CCCNC(N)=N)NC(C)=O)C(=O)NC(CCCNC(N)=N)C(=O)NC1=CC2=C(C=C1)C(C)=CC(=O)O2 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
Avoid freeze/thaw cycles. Protect from light. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
- Fluorogenic tri-peptide substrate for measuring the trypsin-like peptidase activity of the 20S proteasome.
- It is cleaved exclusively at the amide R-AMC bond to release fluorescent product.
- Shows low Km and high specific activity. Excitation: 380nm. Emission: 460nm.
- This substrate is useful for inhibitor screening and kinetic analysis.
Product References
- Assessment of proteasome activity in cell lysates and tissue homogenates using peptide substrates: K.J. Rodgers & R.T. Dean; Int. J. Biochem. Cell Biol. 35, 716 (2003)
- Monitoring activity and inhibition of 26S proteasomes with fluorogenic peptide substrates: A.F. Kisselev & A.L. Goldberg; Methods Enzymol. 398, 364 (2005)
- Importance of the different proteolytic sites of the proteasome and the efficacy of inhibitors varies with the protein substrate: A.F. Kisselev, et al.; J. Biol. Chem. 281, 8582 (2006)
- 5,6,3',4'-Tetrahydroxy-7-methoxyflavone as a novel potential proteasome inhibitor: T.L. Chang, et al.; Planta Med. 76, 987 (2010)
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