AdipoGen Life Sciences

Ac-Arg-Leu-Arg-AMC

CHF 75.00
In stock
AG-CP3-0013-M0011 mgCHF 75.00
AG-CP3-0013-M0055 mgCHF 300.00
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Product Details
Synonyms Ac-RLR-AMC . TFA; Proteasome Substrate
Product Type Chemical
Properties
Formula

C30H46N10O6 . C2HF3O2

MW 642.8 . 114.0
Sequence

Acetyl-Arg-Leu-Arg-7-amido-4-methylcoumarin

CAS 929903-87-7 (free base)
Purity Chemicals ≥97% (HPLC)
Appearance Solid lyophilized powder.
Solubility Soluble in 5% acetic acid.
Identity Determined by MS.
Other Product Data

Use: Add from DMSO stock directly to in vitro or in vivo assay at desired concentration. Typical concentrations range from 10-100 μM. Activity with purified proteasome: Km=78μM.

InChi Key CEEMPRHZPRGAAK-RGRVRPFLSA-N
Smiles CC(C)C[C@H](NC(=O)[C@H](CCCNC(N)=N)NC(C)=O)C(=O)NC(CCCNC(N)=N)C(=O)NC1=CC2=C(C=C1)C(C)=CC(=O)O2
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Protect from light.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description
  • Fluorogenic tri-peptide substrate for measuring the trypsin-like peptidase activity of the 20S proteasome.
  • It is cleaved exclusively at the amide R-AMC bond to release fluorescent product.
  • Shows low Km and high specific activity. Excitation: 380nm. Emission: 460nm.
  • This substrate is useful for inhibitor screening and kinetic analysis.
Product References
  1. Assessment of proteasome activity in cell lysates and tissue homogenates using peptide substrates: K.J. Rodgers & R.T. Dean; Int. J. Biochem. Cell Biol. 35, 716 (2003)
  2. Monitoring activity and inhibition of 26S proteasomes with fluorogenic peptide substrates: A.F. Kisselev & A.L. Goldberg; Methods Enzymol. 398, 364 (2005)
  3. Importance of the different proteolytic sites of the proteasome and the efficacy of inhibitors varies with the protein substrate: A.F. Kisselev, et al.; J. Biol. Chem. 281, 8582 (2006)
  4. 5,6,3',4'-Tetrahydroxy-7-methoxyflavone as a novel potential proteasome inhibitor: T.L. Chang, et al.; Planta Med. 76, 987 (2010)
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