AdipoGen Life Sciences

Boc-Leu-Arg-Arg-AMC

CHF 35.00
In stock
AG-CP3-0014-M0011 mgCHF 35.00
AG-CP3-0014-M0055 mgCHF 110.00
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Product Details
Synonyms Boc-LRR-AMC . AcOH; Proteasome Substrate
Product Type Chemical
Properties
Formula

C33H52N10O7 . C2H4O2

MW 700.8 . 60.1
Sequence

Boc-Leu-Arg-Arg-7-amido-4-methylcoumarin

CAS 109358-46-5 (free base)
Purity Chemicals ≥98%
Appearance Solid lyophilized powder.
Solubility Soluble in 5% acetic acid.
Other Product Data

Use: Add from DMSO stock directly to in vitro or in vivo assay at desired concentration. Typical concentrations range from 10-100 μM.

InChi Key ZYZDREMHVGIZMZ-NYTZCTPBSA-N
Smiles Cl.CC(C)C[C@H](NC(=O)OC(C)(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NC(CCCNC(N)=N)C(=O)NC1=CC2=C(C=C1)C(C)=CC(=O)O2
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Protect from light.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description
  • Fluorogenic tri-peptide substrate for measuring the trypsin-like peptidase activity of the 20S proteasome.
  • Substrate for the Kex2 endopeptidase from S. Cervisiae, which has substrate specificity toward the C-terminal side of LR, PR and RR sequences.
  • Can be cleaved by Kallikrein 5 with a P1 site preference for basic residues (R and K).
  • Excitation: 380nm. Emission: 460nm.
  • This substrate is useful for inhibitor screening and kinetic analysis.
Product References
  1. Proteasome active sites allosterically regulate each other, suggesting a cyclical bite-chew mechanism for protein breakdown: A.F. Kisselev, et al.; Mol. Cell 4, 395 (1999)
  2. Extended peptide-based inhibitors efficiently target the proteasome and reveal overlapping specificities of the catalytic beta-subunits: B.M. Kessler, et al.; Chem. Biol. 8, 913 (2001)
  3. Oligopeptidase B from Trypanosoma evansi. A parasite peptidase that inactivates atrial natriuretic factor in the bloodstream of infected hosts: R.E. Morty, et al.; J. Biol. Chem. 280, 10925 (2005)
  4. Enzymatic analysis of recombinant Japanese encephalitis virus NS2B(H)-NS3pro protease with fluorogenic model peptide substrates: M. Junaid, et al.; PLoS One 7, e36827 (2012)
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