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AdipoGen Life Sciences
Boc-Leu-Arg-Arg-AMC
35
CHF
CHF 35.00
In stock
AG-CP3-0014-M0011 mgCHF 35.00
AG-CP3-0014-M0055 mgCHF 110.00
| Product Details | |
|---|---|
| Synonyms | Boc-LRR-AMC . AcOH; Proteasome Substrate |
| Product Type | Chemical |
| Properties | |
| Formula |
C33H52N10O7 . C2H4O2 |
| MW | 700.8 . 60.1 |
| Sequence |
Boc-Leu-Arg-Arg-7-amido-4-methylcoumarin |
| CAS | 109358-46-5 (free base) |
| Purity Chemicals | ≥98% |
| Appearance | Solid lyophilized powder. |
| Solubility | Soluble in 5% acetic acid. |
| Other Product Data |
Use: Add from DMSO stock directly to in vitro or in vivo assay at desired concentration. Typical concentrations range from 10-100 μM. |
| InChi Key | ZYZDREMHVGIZMZ-NYTZCTPBSA-N |
| Smiles | Cl.CC(C)C[C@H](NC(=O)OC(C)(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NC(CCCNC(N)=N)C(=O)NC1=CC2=C(C=C1)C(C)=CC(=O)O2 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
Avoid freeze/thaw cycles. Protect from light. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
- Fluorogenic tri-peptide substrate for measuring the trypsin-like peptidase activity of the 20S proteasome.
- Substrate for the Kex2 endopeptidase from S. Cervisiae, which has substrate specificity toward the C-terminal side of LR, PR and RR sequences.
- Can be cleaved by Kallikrein 5 with a P1 site preference for basic residues (R and K).
- Excitation: 380nm. Emission: 460nm.
- This substrate is useful for inhibitor screening and kinetic analysis.
Product References
- Proteasome active sites allosterically regulate each other, suggesting a cyclical bite-chew mechanism for protein breakdown: A.F. Kisselev, et al.; Mol. Cell 4, 395 (1999)
- Extended peptide-based inhibitors efficiently target the proteasome and reveal overlapping specificities of the catalytic beta-subunits: B.M. Kessler, et al.; Chem. Biol. 8, 913 (2001)
- Oligopeptidase B from Trypanosoma evansi. A parasite peptidase that inactivates atrial natriuretic factor in the bloodstream of infected hosts: R.E. Morty, et al.; J. Biol. Chem. 280, 10925 (2005)
- Enzymatic analysis of recombinant Japanese encephalitis virus NS2B(H)-NS3pro protease with fluorogenic model peptide substrates: M. Junaid, et al.; PLoS One 7, e36827 (2012)
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