Cookie Policy: This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.
AdipoGen Life Sciences
Suc-Leu-Leu-Val-Tyr-AMC
40
CHF
CHF 40.00
In stock
AG-CP3-0016-M0011 mgCHF 40.00
AG-CP3-0016-M0055 mgCHF 95.00
| Product Details | |
|---|---|
| Synonyms | Suc-LLVY-AMC; Proteasome Substrate; Calpain Substrate, Cathepsin B Substrate |
| Product Type | Chemical |
| Properties | |
| Formula |
C40H53N5O10 |
| MW | 763.9 |
| Sequence |
N-Succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin |
| CAS | 94367-21-2 |
| Purity Chemicals | ≥95% (HPLC) |
| Appearance | Solid lyophilized powder. |
| Solubility | Soluble in DMSO. |
| Other Product Data |
Use: Add from DMSO stock directly to in vitro or in vivo assays at desired concentration. Typical concentrations range from 10-100µM. |
| InChi Key | UVFAEQZFLBGVRM-FEIGSSFXSA-N |
| Smiles | CC(C)C[C@H](NC(=O)CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)NC1=CC2=C(C=C1)C(C)=CC(=O)O2 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
Avoid freeze/thaw cycles. Protect from light. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
- Fluorogenic substrate for measuring the chymotrypsin-like peptidase activity of the 20S proteasome, calpains and other chymotrypsin-like proteases.
- Extinction: 380nm. Emission: 460 nm.
- This substrate is useful for inhibitor screening and kinetic analysis.
Product References
- Comparative specificity and kinetic studies on porcine calpain I and calpain II with naturally occurring peptides and synthetic fluorogenic substrates: T. Sasaki, et al.; J. Biol. Chem. 259, 12489 (1984)
- Kinetic characterization of the chymotryptic activity of the 20S proteasome: R.L. Stein, et al.; Biochemistry 35, 3899 (1996)
- 26S proteasome activity is down-regulated in lung cancer stem-like cells propagated in vitro: J. Pan, et al.; PLoS One 5, e13298 (2010)
- Low cell density induced epithelial-mesenchymal transition in MCF10A cell is regulated by proteasome activity: J. Fu, et al.; J. Oncobiomark. 1, 4 (2013)
- Marchantin M: a novel inhibitor of proteasome induces autophagic cell death in prostate cancer cells: H. Jiang, et al.; Cell Death Dis. 4, e761 (2013)
- Silencing of the ER and Integrative Stress Responses in the Liver of Mice with Error-Prone Translation: J. Moore, et al.; Cells 10, 2856 (2021)
- Analysis of a degron-containing reporter protein GFP-CL1 reveals a role for SUMO1 in cytosolic protein quality control: W. Wang, et al.; J. Biol. Chem. ahead of print (2022)
Related Products
