AdipoGen Life Sciences

Suc-Leu-Leu-Val-Tyr-AMC

CHF 40.00
In stock
AG-CP3-0016-M0011 mgCHF 40.00
AG-CP3-0016-M0055 mgCHF 95.00
More Information
Product Details
Synonyms Suc-LLVY-AMC; Proteasome Substrate; Calpain Substrate, Cathepsin B Substrate
Product Type Chemical
Properties
Formula

C40H53N5O10

MW 763.9
Sequence

N-Succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin

CAS 94367-21-2
Purity Chemicals ≥95% (HPLC)
Appearance Solid lyophilized powder.
Solubility Soluble in DMSO.
Other Product Data

Use: Add from DMSO stock directly to in vitro or in vivo assays at desired concentration. Typical concentrations range from 10-100µM.

InChi Key UVFAEQZFLBGVRM-FEIGSSFXSA-N
Smiles CC(C)C[C@H](NC(=O)CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)NC1=CC2=C(C=C1)C(C)=CC(=O)O2
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Protect from light.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description
  • Fluorogenic substrate for measuring the chymotrypsin-like peptidase activity of the 20S proteasome, calpains and other chymotrypsin-like proteases.
  • Extinction: 380nm. Emission: 460 nm.
  • This substrate is useful for inhibitor screening and kinetic analysis.
Product References
  1. Comparative specificity and kinetic studies on porcine calpain I and calpain II with naturally occurring peptides and synthetic fluorogenic substrates: T. Sasaki, et al.; J. Biol. Chem. 259, 12489 (1984)
  2. Kinetic characterization of the chymotryptic activity of the 20S proteasome: R.L. Stein, et al.; Biochemistry 35, 3899 (1996)
  3. 26S proteasome activity is down-regulated in lung cancer stem-like cells propagated in vitro: J. Pan, et al.; PLoS One 5, e13298 (2010)
  4. Low cell density induced epithelial-mesenchymal transition in MCF10A cell is regulated by proteasome activity: J. Fu, et al.; J. Oncobiomark. 1, 4 (2013)
  5. Marchantin M: a novel inhibitor of proteasome induces autophagic cell death in prostate cancer cells: H. Jiang, et al.; Cell Death Dis. 4, e761 (2013)
  6. Silencing of the ER and Integrative Stress Responses in the Liver of Mice with Error-Prone Translation: J. Moore, et al.; Cells 10, 2856 (2021)
  7. Analysis of a degron-containing reporter protein GFP-CL1 reveals a role for SUMO1 in cytosolic protein quality control:  W. Wang, et al.; J. Biol. Chem. ahead of print (2022)
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