AdipoGen Life Sciences

Z-Leu-Arg-Gly-Gly-AMC

CHF 55.00

In stock

AG-CP3-0023-M0011 mgCHF 55.00

AG-CP3-0023-M0055 mgCHF 130.00

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Product Details
Synonyms	Z-LRGG-AMC . TFA; UCH Substrate
Product Type	Chemical
Properties
Formula	C₃₄H₄₄N₈O₈ . C₂HF₃O₂
MW	692.8 . 114.0
Sequence	Z-Leu-Arg-Gly-Gly-7-amido-4-methylcoumarin
CAS	167698-68-2 (free base)
Purity Chemicals	≥95% (HPLC)
Appearance	Solid lyophilized powder.
Solubility	Soluble in 10% acetic acid.
Other Product Data	Use: Add from DMSO stock directly to in vitro or in vivo assays at desired concentration. Typical concentrations range from 10-100µM.
InChi Key	OHFBLWHOBJRDAB-CCQIZPNASA-N
Smiles	CC(C)C[C@H](NC(=O)OCC1=CC=CC=C1)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NCC(=O)NC1=CC2=C(C=C1)C(C)=CC(=O)O2
Shipping and Handling
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Avoid freeze/thaw cycles. Protect from light.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Description

Fluorogenic tetrapeptide substrate for ubiquitin C-terminal hydrolases (UCHs, e.g. UCHL3) and isopeptidase T.
LRGG is the preferred substrate sequence of the human deSUMOylating enzymes SENP6 and SENP7 [3].
Excitation: 380nm. Emission: 460nm.

Product References

The papain-like protease from the severe acute respiratory syndrome coronavirus is a deubiquitinating enzyme: H.A. Lindner, et al.; J. Virol. 79, 15199 (2005)
Biochemical characterization of USP7 reveals post-translational modification sites and structural requirements for substrate processing and subcellular localization: A. Fernandez-Montalvan, et al.; FEBS J. 274, 4256 (2007)
Activity profiling of human deSUMOylating enzymes (SENPs) with synthetic substrates suggests an unexpected specificity of two newly characterized members of the family: M. Drag, et al.; Biochem. J. 409, 461 (2008)
Crystal structure of the papain-like protease of MERS coronavirus reveals unusual, potentially druggable active-site features: J. Lei, et al.; Antivir. Res. 109, 72 (2014)

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